BBa_K1616002 1 VVD-YN VVD linked to YN155 (YFP Nter split) with double terminator T7 2015-09-16T11:00:00Z 2015-09-22T06:42:24Z Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the N terminal of YFP split(1) Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The part is coding for the homodimer VVD links by an integration of specific sequence to the N terminal of the YFP split. The downstream part of this composite is double T7 terminator (BBa_B0015). So, this part works with BBa_K1616001. In absence of blue-light, the conformation of the VVD photoreceptor will prevent the formation of the complete fluorescent protein while in presence of the light signal the YFP protein will be reconstituted leading to the fast expression of a yellow fluorescence in our bacteria. false false _2033_ 22805 22805 9 true (1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell. 2002;9(4):789???98. false Johanna Chesnel component2471083 1 BBa_K1616020 component2471090 1 BBa_B0015 component2471076 1 BBa_J61100 annotation2471090 1 BBa_B0015 range2471090 1 962 1090 annotation2471076 1 BBa_J61100 range2471076 1 1 12 annotation2471083 1 BBa_K1616020 range2471083 1 21 953 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J61100 1 BBa_J61100 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A {{JCA_Arkin_RBSFamily}} false true _95_ 0 483 95 In stock false N/A true John Anderson BBa_K1616020 1 VVD-YN VVD linked to YN155 (YFP Nter split) 2015-09-17T11:00:00Z 2015-09-18T04:55:03Z Assembly of 3 parts: BBa_K1616014, BBa_K1616018 and BBa_K1616017 without scars See BBa_K1616002. This part have been created in order to have the three parts without scars (VVD,linker and YFP Nterminal). false false _2033_ 22805 22805 9 false No separation between the 3 parts. false Johanna Chesnel component2471073 1 BBa_K1616018 component2471075 1 BBa_K1616017 component2471071 1 BBa_K1616014 annotation2471075 1 BBa_K1616017 range2471075 1 466 933 annotation2471073 1 BBa_K1616018 range2471073 1 451 465 annotation2471071 1 BBa_K1616014 range2471071 1 1 450 BBa_K1616018 1 Linker-JUN Linker JUN of 5 aa 2015-09-17T11:00:00Z 2015-09-18T03:37:41Z Linker used in association with YFP Nterminal split and VVD. This part is a sequence coding for 5 aa useful to link VVD and YFP N terminal split protein. false false _2033_ 22805 22805 9 false Illegal sites have been checked false Johanna Chesnel annotation2470177 1 linker JUN range2470177 1 1 15 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1616014 1 VVD VVD - homodimer photoreceptor 2015-09-17T11:00:00Z 2015-09-18T02:54:35Z VVD was isolated from Neurospora crassa. Among other photoreceptors, Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homo-dimer in response to a blue-light stimulus. The LOV domain, present in VVD, is a small blue-light sensing domain found in prokaryotes, fungi and plants. After blue-light activation, a covalent bond is formed between the co-factor Flavin mononucleotide (FMN) and one of the cysteine residue. This bond leads to a conformational change inducing functions by dissociating the C-terminal a-helix (Ja) and the LOV-core. In VVD, this undock triggers homodimerization (Bilwes, Dunlap, & Crane, 2007; M??ller & Weber, 2013). Contrary to other photoreceptors, VVD is a small protein with 150 amino-acids facilitating accurate molecular design and avoiding steric issues. Moreover, it is a homo-dimer when most of photo-inducible dimers are heterodimers. In addition, the use of VVD is easy; and doesn???t need any addition of co-factors: VVD works with Flavin adenine dinucleotide (FAD) which is already abundant in eukaryote and prokaryote cells (M??ller & Weber, 2013; Nihongaki, Suzuki, Kawano, & Sato, 2014). false false _2033_ 22805 22805 9 false We found illegal sites (PstI) into VVD sequence, those one have been removed. false Johanna Chesnel annotation2470046 1 VVD range2470046 1 1 450 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K1616017 1 YFP-N YN155 - N terminal YFP split 2015-09-17T11:00:00Z 2016-01-21T02:25:38Z This part works with BBa_K16160016 A split protein is a protein whose sequence has been divided into two (or more) different parts. Often used to study protein-protein interactions, the protein can not perform its function until the parts are put back together. For instance, YFP, the yellow-fluorescent protein, will only express fluorescence when its two parts will be reunited. In normal condition, the production of a protein in response to a stimulus can easily reach several hours due to the many steps required for the protein synthesis. By using split-proteins, we are taking advantage of the absence of fluorescence when the two parts are apart. Indeed, the two parts of our split-YFP, when remaining separated, can be produced without being effective. Therefore, the overall process is far less time-consuming. However, to implement a light control on the fluorescence activation, a genetic construction gathering the VVD photoreceptor and our split-YFP has to be engineered. The new alternative approach for the visualization of protein interactiosn has been developed; the biomolecular fluorescence complementation (BiFC) techniques based on the complementation between fragments of fluorescent proteins; fragments of the yellow fluorescent protein (YFP) brought together by the association of two interaction partners fused to the fragments. They noticed that the spectral characteristics of BiFC of YFP were virtually identical to those of intact YFP.(Chang-Deng Hu, 2003) false false _2033_ 4206 22805 9 false Illegal sites have been checked. false Johanna Chesnel annotation2470148 1 YN155 range2470148 1 1 468 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1616014_sequence 1 cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagag BBa_K1616017_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctag BBa_J61100_sequence 1 aaagaggggaca BBa_K1616002_sequence 1 aaagaggggacatactagagcacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1616020_sequence 1 cacaccctgtatgcccctggcggctacgacatcatgggctacctgatccagatcatgaagcggcccaacccccaggtggaactgggccctgtggatacctctgtggccctgatcctgtgcgacctgaagcagaaagacacccccatcgtgtacgcctccgaggccttcctgtacatgaccggctactccaacgccgaggtgctgggccggaactgtagattcctccagtcccctgacggcatggtcaagcctaagtccacccggaaatacgtggactctaacaccatcaacaccatgcggaaggccatcgaccggaacgctgaggtgcaggtggaagtcgtgaacttcaagaagaacggccagcgcttcgtgaatttcctgaccatgatccccgtgcgggacgagacaggcgagtacagatactccatgggcttccagtgcgagacagagagatccatcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggccttcaatgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggcctag BBa_K1616018_sequence 1 agatccatcgccacc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z