LOCUS       BBa_K1616005            1379 bp    DNA     linear       13-May-2024
DEFINITION  Holin/endolysin reversed - RBS and double T7 terminator
ACCESSION   BBa_K1616005
VERSION     BBa_K1616005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     CDS             138..651
                     /label=Endolysin
     CDS             138..651
                     /label=BBa_K1616006
     RBS             660..673
                     /label=RBS Reversed
     RBS             660..673
                     /label=BBa_K1616011
     CDS             682..1357
                     /label=Holin
     CDS             682..1357
                     /label=BBa_K1616007
     RBS             1366..1379
                     /label=RBS Reversed
     RBS             1366..1379
                     /label=BBa_K1616011
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggct ttatagattt ttatacgcgt cccaagtgcc agttctaaac
      181 gttgtaatga ctcgttttgc gcgattaggt gtttgattat accatctact tttagctaag
      241 ttaactgctg cttcatccca gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct
      301 gccacaccgg tttctcccat ttggaaaacc atattaatca atgcacagcg acgaaccgca
      361 tcaagagaat cataaaccgg ttttaattta gcatttctca ggattccgcg aacagcagca
      421 tcaacatcct gattaaagag tttttcagcc tcatcttttg taattacacc attgcaatta
      481 cgcccaatag ctttatctaa ttcagattta gcagcattaa gtgatggact ttttgtaagc
      541 aaatgaccga tgccaatagt gtaatagcct tctgtgtctt tatagatttt aagtctaaga
      601 ccttcatcta tacgtaacat ttcaaatata ttcataatac ctcctaagta ttactagagt
      661 tgtcccctct ttctactaga gtaattattt agcccttcct aatattctgg ccgcctgagc
      721 acatattgat tcaaggcggt cattacttat atgatcattt ctataccagt acatggttat
      781 cgttccagca tagatattat ccaaattaaa atatggacaa ctgtacatgt agtttatttc
      841 gggagtaggc tttttagttg gtaaaaaagc aaattttgag ttggaataat aatgacgtcc
      901 atttaaatga actgtatatt catccatagt tttatcaaca ggatatcctc caagtgattt
      961 ttcacttatt gttgaaggta attttccttc gtatgctata atatcaacaa aatagtttaa
     1021 gtttttaggg cggaaagaat acaccgcact aaagtctgcc tcagatgata tatgaactat
     1081 ctggagttgt tccagggcga cagattcaaa gcgtgcagtt ctttcctttt caataatttc
     1141 actgtatgtt tcatactttg attgcttata gtactcaaag aaactatctc ccctatacca
     1201 aacaatcgcc attataaaca aaagaattac gacagctacc cgggaagcaa gaaccttccc
     1261 ggtagcgtta tctttgaaca agcgatcgag aacaccaaat agaatatcag agggcgaaaa
     1321 tgatattcta ggtgctgcca tagaccctcc ttttaagtac tagagttgtc ccctctttc
//