BBa_K1616027 1 BBa_K1616027 T7 terminator 2015-09-18T11:00:00Z 2015-09-19T11:39:07Z Kyle Hooper, Ph.D, Applications of a smaller, brighter, more versatile luciferase:NanoLuc??? Luciferase Technology, 2012 Shreeram Akilesh, Bioluminescence: Nature???s Bright Idea,2000. T7 terminator of 129 nucleotides false false _2033_ 24639 24639 9 false None. false Marwa Zerhouni BBa_K1616029 1 BBa_K1616029 His Tag - Nanoluc 2015-09-20T11:00:00Z 2015-09-21T02:21:13Z Genomic sequence T7 promoter - His Tag - Nanoluc false false _2033_ 25840 25840 9 false None false Armelle Louisy annotation2476390 1 His Tag range2476390 1 65 82 annotation2476391 1 Nanoluc range2476391 1 89 598 BBa_K1616023 1 NanoLuc T7 promoter - HisTag- NanoLuc - T7 terminator 2015-09-17T11:00:00Z 2015-09-22T07:13:37Z NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence. NanoLuc (Nluc) is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter. NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence. Thermal stable enzyme NanoLuc retains activity following 30 min incubation at 55 ??C. Active over broad pH range NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7. Monomeric enzyme NanoLuc facilitates use as transcriptional reporter or fusion partner. false false _2033_ 22805 22805 9 false Presence of XbaI site. false Johanna Chesnel component2476500 1 BBa_K1616027 component2476496 1 BBa_K1616028 component2476499 1 BBa_K1616029 annotation2476499 1 BBa_K1616029 range2476499 1 20 633 annotation2476496 1 BBa_K1616028 range2476496 1 1 19 annotation2476500 1 BBa_K1616027 range2476500 1 634 762 BBa_K1616028 1 BBa_K1616028 T7 promoter 2015-09-20T11:00:00Z 2015-09-21T10:42:48Z Genomic sequence T7 promoter false false _2033_ 25840 25840 9 false none false Armelle Louisy annotation2476245 1 promoter range2476245 1 1 19 BBa_K1616028_sequence 1 taatacgactcactatagg BBa_K1616023_sequence 1 taatacgactcactatagggagaccacaacggtttccctccaggaataattttgtttaactttaagaaggagatatacatatgcatcatcaccatcaccatggtggggtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgcttaaaagcttgatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat BBa_K1616027_sequence 1 ctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccggat BBa_K1616029_sequence 1 gagaccacaacggtttccctccaggaataattttgtttaactttaagaaggagatatacatatgcatcatcaccatcaccatggtggggtgttcaccctcgaggatttcgttggagattggagacagaccgctggatacaaccttgatcaggttttggagcagggtggtgtgtctagtcttttccagaacctcggagtgtctgtgacccctatccagagaatcgttctctctggtgagaacggactcaagatcgatatccacgtgatcatcccttacgagggactctcaggtgatcagatgggacagatcgagaagattttcaaggtggtgtaccctgttgatgatcaccacttcaaggtgatcctccactacggaactctcgtgatcgatggtgtgaccccaaacatgatcgattacttcggaaggccatacgagggaatcgctgtgttcgatggaaagaagattaccgttaccggaaccctctggaacggaaacaagattatcgatgagaggctcatcaaccctgatggatctctcttgttcagggtgaccatcaacggtgtgactggttggagactttgcgagagaatccttgcttaaaagcttgatccgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z