LOCUS       BBa_K1617001            1314 bp    DNA     linear       27-May-2024
DEFINITION  RBSstrong FliC MCS
ACCESSION   BBa_K1617001
VERSION     BBa_K1617001.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=Start ATG
     misc_feature    448..450
                     /label=M144A
     misc_feature    595..677
                     /label=BBa_K1617010
     misc_feature    595..677
                     /label=MCS
     misc_feature    245..245
                     /label=Delete Xba1 site
     misc_feature    1221..1223
                     /label=M401G
     misc_feature    323..323
                     /label=Delete Pst1 site
     misc_feature    569..569
                     /label=Delete BamHI site
     misc_feature    680..680
                     /label=s_mutation
     misc_feature    731..731
                     /label=Delete Pst1 site
     misc_feature    1034..1034
                     /label=Delete Spe1 site
     misc_feature    1308..1308
                     /label=Delete Pst1 site
     CDS             19..1314
                     /label=BBa_K1617000
ORIGIN
        1 aaagaggaga aatactagat ggcacaagtc attaatacca acagcctctc gctgatcact
       61 caaaataata tcaacaagaa ccagtctgcg ctgtcgagtt ctatcgagcg tctgtcttct
      121 ggcttgcgta ttaacagcgc gaaggatgac gcagcgggtc aggcgattgc taaccgtttc
      181 acctctaaca ttaaaggcct gactcaggcg gcccgtaacg ccaacgacgg tatctccgtt
      241 gcccagacca ccgaaggcgc gctgtccgaa atcaacaaca acttacagcg tgtgcgtgaa
      301 ctgacggtac aggccactac cggcactaac tctgagtctg atctgtcttc tatccaggac
      361 gaaattaaat cccgtctgga tgaaattgac cgcgtatctg gtcagaccca gttcaacggc
      421 gtgaacgtgc tggcaaaaaa tggctccgca aaaatccagg ttggcgcaaa tgataaccag
      481 actatcacta tcgatctgaa gcagattgat gctaaaactc ttggccttga tggttttagc
      541 gttaaaaata acgatacagt taccaccagt gctccagtaa ctgcttttgg tgctggatcc
      601 gccggcgtcg acatgggtgg cagcatgggt ggcagcatga agcttagatc taccggttca
      661 ggcggtacac ctgttcagat tgataatacc gcaggttccg caactgccaa ccttggtgct
      721 gttagcttag taaaactgca agattccaag ggtaatgata ccgatacata tgcgcttaaa
      781 gatacaaatg gcaatcttta cgctgcggat gtgaatgaaa ctactggtgc tgtttctgtt
      841 aaaactatta cctatactga ctcttccggt gccgccagtt ctccaaccgc ggtcaaactg
      901 ggcggagatg atggcaaaac agaagtggtc gatattgatg gtaaaacata cgattctgcc
      961 gatttaaatg gcggtaatct gcaaacaggt ttgactgctg gtggtgaggc tctgactgct
     1021 gttgcaaatg gtaaaaccac ggacccgctg aaagcgctgg acgatgctat cgcatctgta
     1081 gacaaattcc gttcttccct cggtgcggtg caaaaccgtc tggattccgc ggttaccaac
     1141 ctgaacaaca ccactaccaa cctgtctgaa gcgcagtccc gtattcagga cgccgactat
     1201 gcgaccgaag tgtccaatgg ttcgaaagcg cagatcatcc agcaggccgg taactccgtg
     1261 ttggcaaaag ctaaccaggt accgcagcag gttctgtctc tgctgcaagg ttaa
//