LOCUS       BBa_K1617002            1357 bp    DNA     linear       19-May-2024
DEFINITION  Prom106 RBSstrong FliC_MCS
ACCESSION   BBa_K1617002
VERSION     BBa_K1617002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=Start ATG
     misc_feature    491..493
                     /label=M144A
     misc_feature    638..720
                     /label=BBa_K1617010
     misc_feature    638..720
                     /label=MCS
     misc_feature    288..288
                     /label=Delete Xba1 site
     misc_feature    1264..1266
                     /label=M401G
     misc_feature    366..366
                     /label=Delete Pst1 site
     misc_feature    612..612
                     /label=Delete BamHI site
     misc_feature    723..723
                     /label=s_mutation
     misc_feature    774..774
                     /label=Delete Pst1 site
     misc_feature    1077..1077
                     /label=Delete Spe1 site
     misc_feature    1351..1351
                     /label=Delete Pst1 site
     CDS             62..1357
                     /label=BBa_K1617000
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggcacaa gtcattaata ccaacagcct ctcgctgatc actcaaaata atatcaacaa
      121 gaaccagtct gcgctgtcga gttctatcga gcgtctgtct tctggcttgc gtattaacag
      181 cgcgaaggat gacgcagcgg gtcaggcgat tgctaaccgt ttcacctcta acattaaagg
      241 cctgactcag gcggcccgta acgccaacga cggtatctcc gttgcccaga ccaccgaagg
      301 cgcgctgtcc gaaatcaaca acaacttaca gcgtgtgcgt gaactgacgg tacaggccac
      361 taccggcact aactctgagt ctgatctgtc ttctatccag gacgaaatta aatcccgtct
      421 ggatgaaatt gaccgcgtat ctggtcagac ccagttcaac ggcgtgaacg tgctggcaaa
      481 aaatggctcc gcaaaaatcc aggttggcgc aaatgataac cagactatca ctatcgatct
      541 gaagcagatt gatgctaaaa ctcttggcct tgatggtttt agcgttaaaa ataacgatac
      601 agttaccacc agtgctccag taactgcttt tggtgctgga tccgccggcg tcgacatggg
      661 tggcagcatg ggtggcagca tgaagcttag atctaccggt tcaggcggta cacctgttca
      721 gattgataat accgcaggtt ccgcaactgc caaccttggt gctgttagct tagtaaaact
      781 gcaagattcc aagggtaatg ataccgatac atatgcgctt aaagatacaa atggcaatct
      841 ttacgctgcg gatgtgaatg aaactactgg tgctgtttct gttaaaacta ttacctatac
      901 tgactcttcc ggtgccgcca gttctccaac cgcggtcaaa ctgggcggag atgatggcaa
      961 aacagaagtg gtcgatattg atggtaaaac atacgattct gccgatttaa atggcggtaa
     1021 tctgcaaaca ggtttgactg ctggtggtga ggctctgact gctgttgcaa atggtaaaac
     1081 cacggacccg ctgaaagcgc tggacgatgc tatcgcatct gtagacaaat tccgttcttc
     1141 cctcggtgcg gtgcaaaacc gtctggattc cgcggttacc aacctgaaca acaccactac
     1201 caacctgtct gaagcgcagt cccgtattca ggacgccgac tatgcgaccg aagtgtccaa
     1261 tggttcgaaa gcgcagatca tccagcaggc cggtaactcc gtgttggcaa aagctaacca
     1321 ggtaccgcag caggttctgt ctctgctgca aggttaa
//