BBa_K1627004
1
BBa_K1627004
Device to demethylate paraxanthine
2015-09-09T11:00:00Z
2015-09-17T07:30:00Z
The ndmA, ndmC, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.
This part contains genes that allow for the demethylation of paraxanthine. The part contains a synthetic operon consisting of ndmA, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of paraxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of paraxanthine, a growth curve can be made that correlates culture growth with concentration of paraxanthine. Then if the strain is grown in a minimal media with an unknown concentration of paraxanthine, the growth can be compared to the growth curve to calculate the initial concentration of paraxanthine in the media.
false
false
_2044_
22564
22564
9
false
These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.
false
Alejandro Gutierrez
annotation2465939
1
ndmD
range2465939
1
2036
3802
annotation2465946
1
B0034
range2465946
1
3847
3858
annotation2453585
1
J23110
range2453585
1
1
35
annotation2465935
1
ndmA
range2465935
1
69
1124
annotation2465938
1
B0032
range2465938
1
2017
2029
annotation2465947
1
gst9
range2465947
1
3865
4539
annotation2453586
1
K515105
range2453586
1
44
68
annotation2465937
1
ndmC
range2465937
1
1154
2008
annotation2465936
1
B0030
range2465936
1
1133
1147
BBa_K1627004_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagaaatcaaattaaggaggtaagataaatggaacaggcaatcattaatgatgaacgggagtatcttcgccatttctggcatcccgtatgtactgtaactgagcttgaaaaggcgcatccttccagcctcggccccctggccgttaagctgctgaatgaacagctcgttgtcgccaagctaggcgatgagtacgtcgcgatgcgtgatagatgcgctcatcgatcagctaagctttccttgggtacagtaagtggaaaccgcctacagtgcccctatcacggatggcaatatgatacgcatggcgcttgccagctcgtaccagcgtgccccaacagcccaatacccaacaaggctaaagttgatcgcttcgattgcgaagaacgctatggattgatttggattcgattggactctagttttgactgcactgaaattccctactttagtgcagccaacgatcctaggttgcgtattgttatacaagaaccttactggtgggatgcgactgcggaacgtagatgggaaaattttacagatttttctcactttgcattcattcacccaggcacgcttttcgatccaaataatgctgaacctccaattgttccgatggatcgattcaatggtcagtttcggtttgtctacgacacgccagaagatatggctgtcccaaatcaggctccaattggttcattttcgtatacttgcagcatgccgtttgctattaaccttgaagtatccaaatactccagcagttcgctgcatgtgttattcaatgtgtcatgtccggtagacagccacaccacgaaaaactttctgatcttcgctagggagcaatcggacgactcggattatctgcacattgcatttaatgatctcgtcttcgctgaagacaaaccagtaattgagtcccaatggcctaaagacgcgccagcagatgaggtctcagtagtcgcagataaggtatcgatacaatatagaaaatggctgcgggaactaaaagaagctcataaagaaggttcacaagccttccgaagtgctttgttagacccagtcattgaaagcgataggagctacatataatactagagattaaagaggagaaatactagatgtctactgaccaagtaatttttaacgactggcatccagtcgctgctttggaagatgtatctctcgataaacgataccgctgtcgactactaggtcgtacagttagttatgtgaaaacatctgatgctgttaatgctcattgggaagaaagtgcagatgaaattaagaccatccgcgctaaagaaatctatggtcttctgtggctctcctttgccgacaaacccagtgagatgttcgatattgcagagttcaaggaacctgatcgccgaatcgtcagcgctggatctgtgcgcgtaaatgtctcaggactgcgtgctatcgaaaactttctggacatggctcatttcccttttgttcatacggatattttgggtgcagagccactgacggaagttgagccgtataacgtcaactatgatgaaactgttgatgagatcttcgctactgagtgtaagttcccgcaacctaaaggctcagctactgctgtcgagccaattgatatgcagtatatataccgcattacacggccatattccgccattttatataaaacttgcccgcccgaaccgcatagatgggatgccctcggtctgtttattcaacctgtagacgaagattggtgcatagcgcatacaattatgtgctatgtggatgacgttaactcagatcaacaacttcgccatttccagcagacgatttttggccaggacttgatgattcttatcaaccaagttccaaagcgtcttcccctggctgcaagtcgagagagcccagtccgggccgatgtgcttgcaacagcttatcgtcgctggctgcgtgagaaaggtgtgcaatatggtgctctgcgggactaatactagagtcacacaggaaagtactagatgaacaaacttgacgtcaaccagtggtttcctattgctaccactgaagatctcccgaagcgccatgtctttcatgccacgttgttggggcaagaaatggccatctggcgcgatgactctggttcagttaatgcttgggagaaccgctgcccgcatagaggattgcggttgacactgggtgctaataccggtaacgagttgcggtgtcagtatcatggatggacttatgaaagcgggactggtggctgcacttttgtcccagcccatcgcgatgcaccacccccaaatgccgcgcgggttaatacttttcctgtccgcgaaaagcacggctttatctggacgacattaggtcagccgccaggagagcccatttcaatcctcgatgacgctcagcttgtaaacgctgtaaaaacaaatctgcatagcgtagttatagatgctgatattgacggagttgtcagcgtcctacgtcagaatctttcagcgttcatcgatgtgtttggtgcggccagcgctgaagatctgcatttgaaatccatgctgcaagatcgagggattctggtaacaagatcaggctctattgctattcatttttatatgcagcgctcaaccattagtaaatgcgttgtacatgcgcaagtacttactccgggacgtccaggatacgaacttcaaaagaactactcgtatgccatgaacgttatccgcagggcagcagaagctgtagctaccgacttgattagcattacagatatcagcgatcagactatcgaaaagcttgaagtcgttagagaaaacatgactaaggctcctccaacccactatatctgcgaagtggttacgcgtactcaagagacaggtgatattaactcatactggctgaagcctatcggctacccactaccagcattcagtccagggatgcacatcagcatcacaacgccggagggtagcattcgacaatattccctcgtgaacgggcctgacgagcgtgaatccttcatcatcggtgtgaagaaagagattcagtcccgtggcggctccagatcaatgcacgaagatgtgaaggttggaacgcaactaaaagttacacttccgaggaacggttttccactcgtccaaaccagaaaacacccgattctcgtagcaggtggcatcggtatcaccccaattttgtgtatggcacaggctctggatcagcaaggttcatcgtatgaaatacattattttgctcgtgcatttgagcatgttccattccaggatcgactgactgcgttgggcgatcgtttgaatgtgcatcttggcctcggcccagacgagactagagcaaaacttgccgacatcatggagattcataacgcccaagacgtagatgtttacacttgcggcccgcaaccaatgatcgaaactgtatctgctgtcgctcttgctcatggcatcgctgaagagtccatccgatttgaatttttcagtaaaaagaacgatgttcccgtttctgatgaagaatatgaggttgagctcaaaaaaactggtcaaatattcactgtctcgcctggctctacgttgttgcaagcttgtttggacaacgatgttcgtatcgaagcttcttgtgagcagggtgtatgcgggacttgtataactccagtcgtatccggcgatctcgagcatcatgacacttacctttctaagaaagaaagggaaagcggtaagtggatcatgccgtgtgtttcgcgctgcaagtccaaaaaaatcgttctcgatctgtgaggccgggcatcacccacggtatggacgagctgtataaactagagaaagaggagaaatactagatgatcacgctttatgactatgaactctcggggaattgctacaaggtgcggatgctcctttccatcctgaagctaccgtacacgatagagacagtggaatttttcccgtccagggaacataaatcaccggccttcctgcgcatcaatccactcggccagttgccggtattgcgcgatgatgatttcgtgttgcgcgatgcccaggccatcctggtttacctggcgaacaaatacgacaccagtttgacctggtatccgacacatgcgcccgccatcctcggccaggcaaatatgtggctcgcgtttgccgatggtttgaccggctcgatttccgctgcacgtttgcatgatttgtttttttacgatttcaatgcagagcaatgccgtgcccgcgcgcacgaactactgcgcatactggacgaacacctgtgggcggcggaacaaaatggacatcaatggctatgtccggccgccgcacccaccatcgcggatcttgcctgcttcccgtatatcgcattgtcggatgaagccggcgtttcactactcgactacccggcggtacgacgctggctggaccgtgtcaaacgcatccccggattcaccgtcatgtcggggatttttccaacctcagcttccttcgatccggcctcacttgcggaagaaacctgtcaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z