BBa_K1631005 1 BBa_K1631005 Translational unit of Barstar (Barnase immunity protein) 2015-09-13T11:00:00Z 2015-09-14T03:19:43Z Bacillus amyloliquefaciens Barstar is a immunity protein of Barnase, RNase form Bacillus amyloliquefaciens. Barstar blocks the active site of barnase sterically[1]. Rference [1]Buckle, A. M., Schreiber, G., & Fersht, A. R. (1994). Protein-protein recognition: Crystal structural analysis of a barnase-barstar complex at 2.0-. ANG. resolution. Biochemistry, 33(30), 8878-8889. false false _2048_ 23567 23567 9 false none false Yuto Yamanaka annotation2453901 1 BBa_B0034 range2453901 1 1 12 annotation2453902 1 barstar range2453902 1 13 285 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1631007 1 BBa_K1631007 rbs - Barstar - d.term 2015-09-13T11:00:00Z 2015-09-14T04:42:43Z Assembly This part is a composite part of Translational unit of Barstar(BBa_K1631005) and double termnator (BBa_B0015). false false _2048_ 23567 23567 9 false none false Yuto Yamanaka component2453962 1 BBa_B0015 component2453955 1 BBa_K1631005 annotation2453955 1 BBa_K1631005 range2453955 1 1 285 annotation2453962 1 BBa_B0015 range2453962 1 294 422 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1631007_sequence 1 aaagaggagaaaatgaaaaaagcggttatcaacggtgaacagatccgttctatctctgacctgcaccagaccctgaaaaaagaactggcgctgccggaatactacggtgaaaacctggacgcgctgtgggactgcctgaccggttgggttgaatacccgctggttctggaatggcgtcagttcgaacagtctaaacagctgaccgaaaacggtgcggaatctgttctccaggttttccgtgaagcgaaagcggaaggttgcgacatcaccatcatcctgtcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1631005_sequence 1 aaagaggagaaaatgaaaaaagcggttatcaacggtgaacagatccgttctatctctgacctgcaccagaccctgaaaaaagaactggcgctgccggaatactacggtgaaaacctggacgcgctgtgggactgcctgaccggttgggttgaatacccgctggttctggaatggcgtcagttcgaacagtctaaacagctgaccgaaaacggtgcggaatctgttctccaggttttccgtgaagcgaaagcggaaggttgcgacatcaccatcatcctgtcttaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z