LOCUS       BBa_K1631023             485 bp    DNA     linear       07-May-2024
DEFINITION  Plac -rbs- Barstar -d.term
ACCESSION   BBa_K1631023
VERSION     BBa_K1631023.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..75
                     /label=BBa_B0034
     CDS             76..348
                     /label=barstar
     misc_feature    64..348
                     /label=BBa_K1631005
     stem_loop       368..411
                     /label=stem_loop
     misc_feature    357..436
                     /label=BBa_B0010
     terminator      357..436
                     /label=BBa_B0010
     stem_loop       452..471
                     /label=T7 TE
     misc_feature    478..478
                     /label=stop
     polyA_site      472..485
                     /label=polya
     misc_feature    445..485
                     /label=BBa_B0012
     terminator      445..485
                     /label=BBa_B0012
     terminator      357..485
                     /label=BBa_B0015
     misc_feature    64..485
                     /label=BBa_K1631007
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaaatgaa aaaagcggtt atcaacggtg aacagatccg ttctatctct
      121 gacctgcacc agaccctgaa aaaagaactg gcgctgccgg aatactacgg tgaaaacctg
      181 gacgcgctgt gggactgcct gaccggttgg gttgaatacc cgctggttct ggaatggcgt
      241 cagttcgaac agtctaaaca gctgaccgaa aacggtgcgg aatctgttct ccaggttttc
      301 cgtgaagcga aagcggaagg ttgcgacatc accatcatcc tgtcttaata ctagagccag
      361 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      421 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      481 ttata
//