LOCUS       BBa_K1638030            1976 bp    DNA     linear       13-May-2024
DEFINITION  Leucine zipper fused to T25 domain of <i>cyaA</i> with 
            cAMP-induced RFP generator
ACCESSION   BBa_K1638030
VERSION     BBa_K1638030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     CDS             158..832
                     /label=mrfp1
     misc_feature    839..863
                     /label=Help:Barcodes
     CDS             158..838
                     /label=BBa_E1010
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
     misc_feature    140..1000
                     /label=BBa_I13507
     misc_feature    1..1000
                     /label=BBa_K861173
     promoter        1096..1120
                     /label=Lac promoter
     misc_feature    1129..1150
                     /label=Lac operator
     misc_feature    1063..1078
                     /label=CAP binding
     promoter        1009..1150
                     /label=BBa_K1638000
     misc_feature    1159..1164
                     /label=Shine-Dalgarno
     RBS             1151..1169
                     /label=BBa_K1638001
     misc_feature    1851..1856
                     /label=BamHI restriction site
     CDS             1170..1856
                     /label=BBa_K1638002
     misc_feature    1009..1856
                     /label=BBa_K1638003
     misc_feature    1857..1880
                     /label=BBa_K1638016
     CDS             1881..1976
                     /label=BBa_K1638012
     misc_feature    1857..1976
                     /label=BBa_S05273
     misc_feature    1009..1976
                     /label=BBa_K1638013
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat
      181 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga
      241 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt
      301 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg
      361 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc
      421 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac
      481 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa
      541 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga
      601 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa
      661 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca
      721 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta
      781 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg
      841 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagct ggcacgacag
     1021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1141 cggataacaa tttcacacag gaaacagcta tgaccatgca gcaatcgcat caggctggtt
     1201 acgcaaacgc cgccgaccgg gagtctggca tccccgcagc cgtactcgat ggcatcaagg
     1261 ccgtggcgaa ggaaaaaaac gccacattga tgttccgcct ggtcaacccc cattccacca
     1321 gcctgattgc cgaaggggtg gccaccaaag gattgggcgt gcacgccaag tcgtccgatt
     1381 gggggttgca ggcgggctac attcccgtca acccgaatct ttccaaactg ttcggccgtg
     1441 cgcccgaggt gatcgcgcgg gccgacaacg acgtcaacag cagcctggcg catggccata
     1501 ccgcggtcga cctgacgctg tcgaaagagc ggcttgacta tctgcggcaa gcgggcctgg
     1561 tcaccggcat ggccgatggc gtggtcgcga gcaaccacgc aggctacgag cagttcgagt
     1621 ttcgcgtgaa ggaaacctcg gacgggcgct atgccgtgca gtatcgccgc aagggcggcg
     1681 acgatttcga ggcggtcaag gtgatcggca atgccgccgg tattccactg acggcggata
     1741 tcgacatgtt cgccattatg ccgcatctgt ccaacttccg cgactcggcg cgcagttcgg
     1801 tgaccagcgg cgattcggtg accgattacc tggcgcgcac gcggcgggct ggatccgaaa
     1861 atttgtattt tcaatctggt atgaaacagc tggaagacaa agttgaagaa ctgctgtcca
     1921 aaaactacca cctggaaaac gaagttgctc gtctgaaaaa actggttggt gaacgt
//