BBa_K1639008
1
BBa_K1639008
Tev Protease, single S219V mutation in the internal cleavage site
2015-09-12T11:00:00Z
2015-09-18T07:12:27Z
Tobacco etch virus (TEV).
TEV protease (also called Tobacco Etch Virus nuclear inclusion a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV).The consensus for these native cut sites is ENLYFQ\S where ???\??? denotes the cleaved peptide bond. One of the main uses of this protein is for removing affinity tags from purified proteins. The reason for the use of TEV protease as a biochemical tool is its high sequence specificity. This specificity allows for the controlled cleavage of proteins when the preference sequence is inserted into flexible loops. It also makes it relatively non-toxic in vivo as the recognized sequence scarcely occurs in proteins.[1]
false
false
_2056_
24789
20835
9
false
This part contains an additional BamHI restriction site at beginning of Tev Protease sequence and a XhoI restriction site at the end.
false
Mustafa Yılmaz
annotation2453264
1
S219V
range2453264
1
666
668
annotation2453252
1
ATG Start Codon
range2453252
1
9
11
annotation2453254
1
Stop Codon
range2453254
1
738
740
annotation2453253
1
Tev Protease
range2453253
1
12
737
BBa_K1639008_sequence
1
gggatccgatgggcgagtcgctgttcaagggcccgcgtgactacaacccgatttcttctacgatttgtcatttgacaaatgaaagtgatggtcataccaccagcctctacggcattggattcggcccgtttattattacgaacaagcatttatttcgtcgtaataacggcaccctgctcgtgcaatcactccatggcgttttcaaagtcaaaaataccacgacccttcaacagcaccttattgatggtcgtgacatgatcattatccgcatgccgaaagactttccgccatttccgcagaagctcaagtttcgcgaacctcagcgcgaagaacgtatttgcttagtaactaccaattttcagactaaaagtatgagcagcatggtgtccgatacttcctgtacctttccatcatcagatggcattttttggaaacattggattcaaaccaaagacggtcagtgcggtagccctctggtatctacacgcgatggttttattgttggtattcatagcgcttcgaattttactaacaccaacaactactttacgtccgtgccaaaaaacttcatggaactgctgacaaaccaggaagcgcaacagtgggtctcaggttggcgtctgaatgcggattccgttttgtggggtggccacaaggtttttatggtgaaaccggaggaacctttccagccggtcaaggaagccacccagctgatgaatgaactggtgtatagccaataataactcgagt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z