LOCUS       BBa_K1641037            3338 bp    DNA     linear       15-May-2024
DEFINITION  Inductive invertase generating vector: pInv-gen-Flp-EGFP-ssra
ACCESSION   BBa_K1641037
VERSION     BBa_K1641037.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1230
                     /label=B0034
     misc_feature    1231..1236
                     /label=scar
     misc_feature    2505..2529
                     /label=flexible chain
     CDS             1237..2504
                     /label=Flp (without stop)
     CDS             2530..3288
                     /label=EGFP-ssra(M0051)
     misc_feature    1219..3291
                     /label=BBa_K1641005
     misc_feature    3300..3338
                     /label=B1006
     stem_loop       3309..3330
                     /label=modified thr terminator
     polyA_site      3331..3338
                     /label=PolyA
     polyA_site      3300..3308
                     /label=PolyA
     terminator      3300..3338
                     /label=BBa_B1006
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa actagaatga gccagtttgg catattatgt
     1261 aaaacaccac ctaaggtcct ggttcgtcag tttgtggaaa ggtttgaaag accttcaggg
     1321 gaaaaaatag catcatgtgc tgctgaacta acctatttat gttggatgat tactcataac
     1381 ggaacagcaa tcaagagagc cacattcatg agctataata ctatcataag caattcgctg
     1441 agtttcgata ttgtcaacaa atcactccag tttaaataca agacgcaaaa agcaacaatt
     1501 ctggaagcct cattaaagaa attaattcct gcttgggaat ttacaattat tccttacaat
     1561 ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt acagttcgaa
     1621 tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa agcacttcta
     1681 agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt tgagtatacc
     1741 tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac tttcatcaat
     1801 tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt agtccaaaat
     1861 aagtatctgg gagtaataat ccagtgttta gtgacagaga caaagacaag cgttagtagg
     1921 cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt ggatgaattt
     1981 ttgagaaatt ctgaaccagt cctaaaacga gtaaatagga ccggcaattc ttcaagcaac
     2041 aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaacaa ggctttgaag
     2101 aaaaatgcgc cttatccaat ctttgctata aagaatggcc caaaatctca cattggaaga
     2161 catttgatga cctcatttct gtcaatgaag ggcctaacgg agttgactaa tgttgtggga
     2221 aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca tcagataaca
     2281 gcaatacctg atcactactt cgcgctagtt tctcggtact atgcatatga tccaatatca
     2341 aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca gcatatagaa
     2401 cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg gataatatca
     2461 caggaggtac tagactacct ttcatcctac ataaatagac gcataggagc tgatacaggt
     2521 agcggaggta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaaggctg ctaacgacga aaactacaac tacgctgacg cttcttaata atactagaga
     3301 aaaaaaaacc ccgcccctga cagggcgggg tttttttt
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