LOCUS       BBa_K1641043            2972 bp    DNA     linear       14-May-2024
DEFINITION  Inductive invertase generating vector: pInv-gen-Cre-EGFP
ACCESSION   BBa_K1641043
VERSION     BBa_K1641043.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1230
                     /label=B0034
     CDS             1237..2178
                     /label=Cre (in fusion)
     misc_feature    2179..2202
                     /label=flexible chain
     CDS             2203..2922
                     /label=EGFP (in fusion)
     misc_feature    1219..2925
                     /label=BBa_K1641042
     misc_feature    2934..2972
                     /label=B1006
     stem_loop       2943..2964
                     /label=modified thr terminator
     polyA_site      2965..2972
                     /label=PolyA
     polyA_site      2934..2942
                     /label=PolyA
     terminator      2934..2972
                     /label=BBa_B1006
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa actagaatgt tcagggatcg ccaggcgttt
     1261 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
     1321 ttgaataacc ggaaatggtt ttccgcagaa cctgaagatg ttcgcgatta tcttctatat
     1381 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
     1441 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
     1501 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     1561 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     1621 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     1681 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     1741 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     1801 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     1861 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     1921 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     1981 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     2041 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     2101 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     2161 ctgctggaag atggcgatgg agctgataca ggtagcggag gtatggtgag caagggcgag
     2221 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2281 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2341 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     2401 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     2461 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2521 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2581 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2641 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2701 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2761 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2821 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2881 gccgccggga tcactctcgg catggacgag ctgtacaagt aataatacta gagaaaaaaa
     2941 aaccccgccc ctgacagggc ggggtttttt tt
//