LOCUS       BBa_K1642000            2648 bp    DNA     linear       01-Jun-2024
DEFINITION  PcpcG2-Codon-optimized Halorhodopsin
ACCESSION   BBa_K1642000
VERSION     BBa_K1642000.1  
FEATURES             Location/Qualifiers
     promoter        1..261
                     /label=BBa_K1642002
     RBS             270..289
                     /label=BBa_K1642004
     misc_feature    1169..1186
                     /label=His-tag
     CDS             296..1168
                     /label=Halorhodopsin
     CDS             296..1186
                     /label=BBa_K1642001
     stem_loop       1202..1221
                     /label=T7 TE
     misc_feature    1228..1228
                     /label=stop
     polyA_site      1222..1235
                     /label=polya
     misc_feature    1195..1235
                     /label=BBa_B0012
     terminator      1195..1235
                     /label=BBa_B0012
     promoter        1244..1832
                     /label=PcpcB
     CDS             1833..2645
                     /label=KanR
     misc_feature    1244..2648
                     /label=BBa_K1642003
ORIGIN
        1 tcctccacta aaagaattct catagcccat tgtgcttttc tctatcaacc tcagcttacc
       61 tgaaggggtg aacaggtctg ggttaattca tgttgcgaaa tgtaacagtt ttagtcgcat
      121 cagctaactt tccgatttct ttacgatttt ctcccccttt tcttcaattt tactttgtta
      181 ggatcgcatt tttaatgcca acacatacca gttattggct ggacattaaa caacttttaa
      241 gtttaattac taactttaca ttactagagt ataagtagga gataaaaact actagatgac
      301 tgaaaccttg ccccccgtta ccgagtccgc cgttgcctta caagccgaag tgacccagcg
      361 ggaattgttc gaatttgtgt taaatgatcc cttgttggct tcctccttgt atattaatat
      421 tgccttagcc ggcttatcca ttttgttgtt tgtgttcatg acccggggct tggatgatcc
      481 tcgggccaag ttgattgccg tgtccaccat cttagttccc gtggtttcca ttgcctccta
      541 caccggcttg gccagtggct tgaccatttc cgtgttggag atgcctgccg gccattttgc
      601 tgaaggcagt tccgtgatgt tgggcggcga agaggtggat ggcgtggtga ccatgtgggg
      661 ccggtacttg acctgggcct tgtccacccc catgatcttg ttggccttgg gcttgttggc
      721 cggttccaac gccactaaat tgttcaccgc catcaccttt gacatcgcca tgtgcgtgac
      781 cggtttggcc gccgctttga ccacctcctc ccacttgatg cgctggtttt ggtatgccat
      841 ctcctgcgcc tgtttcttgg tggtgttgta catcttgttg gtggaatggg cccaagatgc
      901 caaagccgcc ggcaccgccg atatgtttaa caccttgaaa ttattgaccg tggtgatgtg
      961 gttgggctac cctattgtgt gggccttggg cgtggaaggc attgccgttt tgcccgtggg
     1021 tgttacctcc tggggctact cctttttgga tattgtggcc aaatacattt ttgccttttt
     1081 gttgttgaac tatttgacct ccaatgaatc cgtggtgtcc ggctccattt tggatgttcc
     1141 ctccgccagt ggcactcccg ccgatgatca tcatcatcac catcattact agagtcacac
     1201 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggtggtat aaacgcagaa
     1261 aggcccctat acccacctgt agagaagagt ccctgaatat caaaatggtg ggataaaaag
     1321 ctcaaaaagg aaagtaggct gtggttccct aggcaacagt cttccctacc ccactggaaa
     1381 ctaaaaaaac gagaaaagtt cgcaccgaac atcaattgca taattttagc cctaaaacat
     1441 aagctgaacg aaactggttg tcttcccttc ccaatccagg acaatctgag aatcccctgc
     1501 aacattactt aacaaaaaag caggaataaa attaacaaga tgtaacagac ataagtccca
     1561 tcaccgttgt ataaagttaa ctgtgggatt gcaaaagcat tcaagcctag gcgctgagct
     1621 gtttgagcat cccggtggcc cttgtcgctg cctccgtgtt tctccctgga tttatttagg
     1681 taatatctct cataaatccc cgggtagtta acgaaagtta atggagatca gtaacaataa
     1741 ctctagggtc attactttgg actccctcag tttatccggg ggaattgtgt ttaagaaaat
     1801 cccaactcat aaagtcaagt aggagattaa ttatgagcca tattcaacgg gaaacgtctt
     1861 gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     1921 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc
     1981 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2041 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2101 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat
     2161 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2221 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgaccgcgta tttcgtctcg
     2281 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2341 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     2401 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     2461 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     2521 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     2581 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     2641 ttttctaa
//