LOCUS       BBa_K1648004            2770 bp    DNA     linear       18-May-2024
DEFINITION  Insertion Kit for Fusing Protein of Interest to Magnetosome 
            Membrane
ACCESSION   BBa_K1648004
VERSION     BBa_K1648004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     misc_feature    63..74
                     /label=BBa_B0034
     misc_feature    67..70
                     /label=conserved
     misc_feature    1..74
                     /label=BBa_J13002
     misc_feature    81..455
                     /label=mamC
     misc_feature    81..455
                     /label=BBa_K1648007
     misc_feature    464..543
                     /label=BBa_B0010
     stem_loop       475..518
                     /label=stem_loop
     misc_feature    552..592
                     /label=BBa_B0012
     stem_loop       559..578
                     /label=T7 TE
     polyA_site      579..592
                     /label=polya
     misc_feature    585..585
                     /label=stop
     misc_feature    464..592
                     /label=BBa_B0015
ORIGIN
        1 actagtctgc agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
       61 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      121 gtgggccttt ctgcgtttat agggggtacc tccggcaaaa aagggcaagg tgtcaccacc
      181 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
      241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
      421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1141 tgagtaaact tggtctgaca gctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt
     1201 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     1261 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1321 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1381 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1441 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     1501 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     1561 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     1621 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1681 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     1741 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1801 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     1861 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     1921 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1981 tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata
     2041 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     2101 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
     2161 cgaacttttg ctgagttgaa ggatcagctc gagtgccacc tgacgtctaa gaaaccatta
     2221 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     2281 aatccttagc tttcgctaag gatgatttct ccctatcagt gatagagatt gacatcccta
     2341 tcagtgatag agatactgag cactactaga gaaagaggag aaaatgagct ttcaacttgc
     2401 gccgtacttg gcgaaatccg tccctggaat cggcattctc ggcggcattg tcggtggcgc
     2461 cgccgccctt gccaagaatg cccgcctttt gaaggacaag cagataaccg gcacagaagc
     2521 ggccatcgac accggcaagg aagccgccgg cgccgggctt gccaccgctt tctccgccgt
     2581 cgccgccacc gccgtcggcg gtgggttggt ggtctcgttg ggagccgccc taatcgccgg
     2641 cgtcgccgcc aaatacgcct gggacctggg tgtcgatttc atcgagaagg aattgcgtca
     2701 cggcaagtcc gccgaggcga cagcgtccga cgaagacatt ctgagggaag aattggccga
     2761 attctctaga
//