BBa_K1648004
1
BBa_K1648004
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
2015-07-16T11:00:00Z
2015-09-23T11:07:34Z
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome
[[File:K1648004_Map.png|350px|thumb|right|Construct Map for K1648004]]
Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome.
Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' except the mamC operon is designed for '''homologous recombination'''.
Part containing this template ([http://parts.igem.org/Part:BBa_K1648002 K1648002]) together with another part containing mamC ([http://parts.igem.org/Part:BBa_K1648XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''.
false
false
_2065_
26823
26823
9
false
In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from '''mamGFDC''', '''mamYXZ-ftsZ''' and '''mms6-mmsF-mms35-mms48''' ('''mamGC''', '''mamXY''' and '''mms6 operons''') except the mamC operon as flanking sequences. Fragments are joined via overlapping PCR.
[[File:K1648004_History.png|900px]]
false
Leung King Pong
annotation2463385
1
TetR 1
range2463385
1
1
19
annotation2463400
1
stop
range2463400
1
585
585
annotation2463395
1
BBa_B0010
range2463395
1
464
543
annotation2463394
1
BBa_K1648007
range2463394
1
81
455
annotation2463391
1
conserved
range2463391
1
67
70
annotation2463387
1
-35
range2463387
1
20
25
annotation2463398
1
T7 TE
range2463398
1
559
578
annotation2463392
1
BBa_J13002
range2463392
1
1
74
annotation2463401
1
BBa_B0015
range2463401
1
464
592
annotation2463389
1
-10
range2463389
1
43
48
annotation2463396
1
stem_loop
range2463396
1
475
518
annotation2463393
1
mamC
range2463393
1
81
455
annotation2463390
1
BBa_B0034
range2463390
1
63
74
annotation2463397
1
BBa_B0012
range2463397
1
552
592
annotation2463399
1
polya
range2463399
1
579
592
annotation2463388
1
TetR 2
range2463388
1
26
44
annotation2463386
1
BBa_R0040
range2463386
1
1
54
BBa_K1648004_sequence
1
actagtctgcagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagggggtacctccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaaatgagctttcaacttgcgccgtacttggcgaaatccgtccctggaatcggcattctcggcggcattgtcggtggcgccgccgcccttgccaagaatgcccgccttttgaaggacaagcagataaccggcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggccgaattctctaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z