BBa_K1648004 1 BBa_K1648004 Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane 2015-07-16T11:00:00Z 2015-09-23T11:07:34Z Magnetospirillum gryphiswaldense MSR-1 v2, complete genome [[File:K1648004_Map.png|350px|thumb|right|Construct Map for K1648004]] Consist of six fragments from magnetosome island of ''Magnetospirillum gryphiswaldense'' genome. Due to potential difficulty in introducing the large magnetosome formation operons into ''Azotobacter vinelandii'', a "template" consists of flanking sequences from '''mamXY, mamGFDC and mms6 operons''' except the mamC operon is designed for '''homologous recombination'''. Part containing this template ([http://parts.igem.org/Part:BBa_K1648002 K1648002]) together with another part containing mamC ([http://parts.igem.org/Part:BBa_K1648XXX K1648XXX]) is introduced into ''A. vinelandii'' genome via '''random integration''' before full operon sequences to be transformed into ''A. vinelandii''. false false _2065_ 26823 26823 9 false In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from '''mamGFDC''', '''mamYXZ-ftsZ''' and '''mms6-mmsF-mms35-mms48''' ('''mamGC''', '''mamXY''' and '''mms6 operons''') except the mamC operon as flanking sequences. Fragments are joined via overlapping PCR. [[File:K1648004_History.png|900px]] false Leung King Pong annotation2463385 1 TetR 1 range2463385 1 1 19 annotation2463400 1 stop range2463400 1 585 585 annotation2463395 1 BBa_B0010 range2463395 1 464 543 annotation2463394 1 BBa_K1648007 range2463394 1 81 455 annotation2463391 1 conserved range2463391 1 67 70 annotation2463387 1 -35 range2463387 1 20 25 annotation2463398 1 T7 TE range2463398 1 559 578 annotation2463392 1 BBa_J13002 range2463392 1 1 74 annotation2463401 1 BBa_B0015 range2463401 1 464 592 annotation2463389 1 -10 range2463389 1 43 48 annotation2463396 1 stem_loop range2463396 1 475 518 annotation2463393 1 mamC range2463393 1 81 455 annotation2463390 1 BBa_B0034 range2463390 1 63 74 annotation2463397 1 BBa_B0012 range2463397 1 552 592 annotation2463399 1 polya range2463399 1 579 592 annotation2463388 1 TetR 2 range2463388 1 26 44 annotation2463386 1 BBa_R0040 range2463386 1 1 54 BBa_K1648004_sequence 1 actagtctgcagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagggggtacctccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagctcgagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaaatgagctttcaacttgcgccgtacttggcgaaatccgtccctggaatcggcattctcggcggcattgtcggtggcgccgccgcccttgccaagaatgcccgccttttgaaggacaagcagataaccggcacagaagcggccatcgacaccggcaaggaagccgccggcgccgggcttgccaccgctttctccgccgtcgccgccaccgccgtcggcggtgggttggtggtctcgttgggagccgccctaatcgccggcgtcgccgccaaatacgcctgggacctgggtgtcgatttcatcgagaaggaattgcgtcacggcaagtccgccgaggcgacagcgtccgacgaagacattctgagggaagaattggccgaattctctaga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z