LOCUS       BBa_K1653019            1118 bp    DNA     linear       10-May-2024
DEFINITION  m-ispA.dev
ACCESSION   BBa_K1653019
VERSION     BBa_K1653019.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..981
                     /label=m-ispA
     CDS             82..981
                     /label=BBa_K1653004
     stem_loop       1001..1044
                     /label=stem_loop
     misc_feature    990..1069
                     /label=BBa_B0010
     terminator      990..1069
                     /label=BBa_B0010
     stem_loop       1085..1104
                     /label=T7 TE
     misc_feature    1111..1111
                     /label=stop
     polyA_site      1105..1118
                     /label=polya
     misc_feature    1078..1118
                     /label=BBa_B0012
     terminator      1078..1118
                     /label=BBa_B0012
     terminator      990..1118
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggacttt ccgcagcaac tcgaagcctg cgttaagcag
      121 gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc
      181 gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat
      241 gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt
      301 gagtgtatcc acgcttactt tttaattcat gatgatttac cggcaatgga tgatgacgat
      361 ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct
      421 ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg
      481 tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga
      541 atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg
      601 cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt
      661 gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag
      721 agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca
      781 acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt
      841 ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg
      901 ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac
      961 atcatccagc gtaataaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1081 cactggctca ccttcgggtg ggcctttctg cgtttata
//