LOCUS       BBa_K1658004            2614 bp    DNA     linear       28-Apr-2024
DEFINITION  Limonene Synthase LS
ACCESSION   BBa_K1658004
VERSION     BBa_K1658004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    44..46
                     /label=SacI
     RBS             47..53
                     /label=rbs
     RBS             44..53
                     /label=BBa_J15001
     CDS             60..1730
                     /label=(+) limonene synthase coding region
     misc_feature    60..62
                     /label=start
     misc_feature    1725..1730
                     /label=stop
     CDS             60..1730
                     /label=BBa_I742110
     misc_feature    44..1730
                     /label=BBa_I742111
     RBS             1739..1751
                     /label=RBS-3\Weak
     RBS             1745..1748
                     /label=RBS
     misc_feature    1739..1751
                     /label=BBa_B0032
     RBS             1739..1751
                     /label=BBa_B0032
     CDS             1758..2477
                     /label=GFP protein
     CDS             1758..2477
                     /label=BBa_E0040
     stem_loop       2497..2540
                     /label=stem_loop
     misc_feature    2486..2565
                     /label=BBa_B0010
     terminator      2486..2565
                     /label=BBa_B0010
     stem_loop       2581..2600
                     /label=T7 TE
     misc_feature    2607..2607
                     /label=stop
     polyA_site      2601..2614
                     /label=polya
     misc_feature    2574..2614
                     /label=BBa_B0012
     terminator      2574..2614
                     /label=BBa_B0012
     misc_feature    1739..2614
                     /label=BBa_E0240
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagctcaagg aggtactaga
       61 tgcgtcgttc agcaaactac caaccttcaa tttgggacca cgattttttg cagtcattga
      121 atagcaacta tacggatgaa gcatacaaaa gacgagcaga agagctgagg ggaaaagtga
      181 agatagcgat taaggatgta atcgagcctc tggatcagtt ggagctgatt gataacttgc
      241 aaagacttgg attggctcat cgttttgaga ctgagattag gaacatattg aataatatct
      301 acaacaataa taaagattat aattggagaa aagaaaatct gtatgcaacc tcccttgagt
      361 ttagactact tagacaacat ggctatcctg tttctcaaga ggttttcaat ggttttaaag
      421 acgaccaggg aggcttcatt tgtgatgatt tcaagggaat actgagcttg catgaagctt
      481 cgtattacag cttagaagga gaaagcatca tggaggaggc ctggcaattt accagcaaac
      541 atcttaaaga agtgatgatc agcaagaaca tggaagagga tgtatttgta gcagaacaag
      601 cgaagcgtgc actggaactg cctctgcatt ggaaagtgcc aatgttagag gcaaggtggt
      661 tcatacacat ttatgagaga agagaggaca agaaccacct tttacttgaa ctggctaaga
      721 tggagtttaa cactttgcag gcaatttacc aggaagaact aaaagaaatt tcagggtggt
      781 ggaaggatac aggtcttgga gagaaattga gctttgcgag gaacaggttg gtagcgtcct
      841 tcttatggag catggggatc gcgtttgagc ctcaattcgc ctattgtcgg agagtgctca
      901 caatctcgat agccctaatt acagtgattg atgacattta tgatgtctat ggaacattgg
      961 atgaacttga gatattcact gatgctgttg agaggtggga catcaattat gctttgaagc
     1021 accttccggg ctatatgaaa atgtgttttc ttgcgcttta caactttgtt aatgaatttg
     1081 cttattacgt tctcaaacaa caggattttg atttgcttct gagcataaaa aatgcatggc
     1141 ttggcttaat acaagcctac ttggtggagg cgaaatggta ccatagcaag tacacaccga
     1201 aactggaaga atacttggaa aatggattgg tatcaataac gggcccttta attataacga
     1261 tttcatatct ttctggtaca aatccaatca ttaagaagga actggaattt ctggaaagta
     1321 atccagatat agttcactgg tcatccaaga ttttccgtct gcaagatgat ttgggaactt
     1381 catcggacga gatacagaga ggggatgttc cgaaatcaat ccagtgttac atgcatgaaa
     1441 ctggtgcctc agaggaagtt gctcgtcaac acatcaagga tatgatgaga cagatgtgga
     1501 agaaggtgaa tgcatacaca gccgataaag actctccctt gactggaaca actactgagt
     1561 tcctcttgaa tcttgtgaga atgtcccatt ttatgtatct acatggagat gggcatggtg
     1621 ttcaaaacca agagactatc gatgtcggtt ttacattgct ttttcagccc attcccttgg
     1681 aggacaaaca catggctttc acagcatctc ctggcaccaa aggctaataa tactagagtc
     1741 acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1801 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1861 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1921 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1981 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     2041 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     2101 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     2161 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     2221 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     2281 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     2341 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     2401 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     2461 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     2521 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     2581 ggctcacctt cgggtgggcc tttctgcgtt tata
//