LOCUS       BBa_K1658005            2571 bp    DNA     linear       29-May-2024
DEFINITION  Limonane + GFP regulator
ACCESSION   BBa_K1658005
VERSION     BBa_K1658005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     RBS             4..10
                     /label=rbs
     RBS             1..10
                     /label=BBa_J15001
     CDS             17..1687
                     /label=(+) limonene synthase coding region
     misc_feature    17..19
                     /label=start
     misc_feature    1682..1687
                     /label=stop
     CDS             17..1687
                     /label=BBa_I742110
     misc_feature    1..1687
                     /label=BBa_I742111
     RBS             1696..1708
                     /label=RBS-3\Weak
     RBS             1702..1705
                     /label=RBS
     misc_feature    1696..1708
                     /label=BBa_B0032
     RBS             1696..1708
                     /label=BBa_B0032
     CDS             1715..2434
                     /label=GFP protein
     CDS             1715..2434
                     /label=BBa_E0040
     stem_loop       2454..2497
                     /label=stem_loop
     misc_feature    2443..2522
                     /label=BBa_B0010
     terminator      2443..2522
                     /label=BBa_B0010
     stem_loop       2538..2557
                     /label=T7 TE
     misc_feature    2564..2564
                     /label=stop
     polyA_site      2558..2571
                     /label=polya
     misc_feature    2531..2571
                     /label=BBa_B0012
     terminator      2531..2571
                     /label=BBa_B0012
     misc_feature    1696..2571
                     /label=BBa_E0240
ORIGIN
        1 ctcaaggagg tactagatgc gtcgttcagc aaactaccaa ccttcaattt gggaccacga
       61 ttttttgcag tcattgaata gcaactatac ggatgaagca tacaaaagac gagcagaaga
      121 gctgagggga aaagtgaaga tagcgattaa ggatgtaatc gagcctctgg atcagttgga
      181 gctgattgat aacttgcaaa gacttggatt ggctcatcgt tttgagactg agattaggaa
      241 catattgaat aatatctaca acaataataa agattataat tggagaaaag aaaatctgta
      301 tgcaacctcc cttgagttta gactacttag acaacatggc tatcctgttt ctcaagaggt
      361 tttcaatggt tttaaagacg accagggagg cttcatttgt gatgatttca agggaatact
      421 gagcttgcat gaagcttcgt attacagctt agaaggagaa agcatcatgg aggaggcctg
      481 gcaatttacc agcaaacatc ttaaagaagt gatgatcagc aagaacatgg aagaggatgt
      541 atttgtagca gaacaagcga agcgtgcact ggaactgcct ctgcattgga aagtgccaat
      601 gttagaggca aggtggttca tacacattta tgagagaaga gaggacaaga accacctttt
      661 acttgaactg gctaagatgg agtttaacac tttgcaggca atttaccagg aagaactaaa
      721 agaaatttca gggtggtgga aggatacagg tcttggagag aaattgagct ttgcgaggaa
      781 caggttggta gcgtccttct tatggagcat ggggatcgcg tttgagcctc aattcgccta
      841 ttgtcggaga gtgctcacaa tctcgatagc cctaattaca gtgattgatg acatttatga
      901 tgtctatgga acattggatg aacttgagat attcactgat gctgttgaga ggtgggacat
      961 caattatgct ttgaagcacc ttccgggcta tatgaaaatg tgttttcttg cgctttacaa
     1021 ctttgttaat gaatttgctt attacgttct caaacaacag gattttgatt tgcttctgag
     1081 cataaaaaat gcatggcttg gcttaataca agcctacttg gtggaggcga aatggtacca
     1141 tagcaagtac acaccgaaac tggaagaata cttggaaaat ggattggtat caataacggg
     1201 ccctttaatt ataacgattt catatctttc tggtacaaat ccaatcatta agaaggaact
     1261 ggaatttctg gaaagtaatc cagatatagt tcactggtca tccaagattt tccgtctgca
     1321 agatgatttg ggaacttcat cggacgagat acagagaggg gatgttccga aatcaatcca
     1381 gtgttacatg catgaaactg gtgcctcaga ggaagttgct cgtcaacaca tcaaggatat
     1441 gatgagacag atgtggaaga aggtgaatgc atacacagcc gataaagact ctcccttgac
     1501 tggaacaact actgagttcc tcttgaatct tgtgagaatg tcccatttta tgtatctaca
     1561 tggagatggg catggtgttc aaaaccaaga gactatcgat gtcggtttta cattgctttt
     1621 tcagcccatt cccttggagg acaaacacat ggctttcaca gcatctcctg gcaccaaagg
     1681 ctaataatac tagagtcaca caggaaagta ctagatgcgt aaaggagaag aacttttcac
     1741 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
     1801 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
     1861 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
     1921 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
     1981 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
     2041 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     2101 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     2161 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
     2221 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     2281 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2341 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2401 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     2461 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2521 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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