LOCUS BBa_K1668008 9672 bp DNA linear 18-May-2024 DEFINITION tcdA1-device ACCESSION BBa_K1668008 VERSION BBa_K1668008.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1215..1218 /label=conserved RBS 1211..1222 /label=BBa_B0034 CDS 1223..8803 /label=BBa_K1668005 misc_feature 8808..8811 /label=conserved RBS 8804..8815 /label=BBa_B0034 CDS 8822..9532 /label=mCherry misc_feature 9173..9173 /label=C->T (removing PstI site) misc_feature 8822..9535 /label=BBa_J06504 stem_loop 9555..9598 /label=stem_loop misc_feature 9544..9623 /label=BBa_B0010 terminator 9544..9623 /label=BBa_B0010 stem_loop 9639..9658 /label=T7 TE misc_feature 9665..9665 /label=stop polyA_site 9659..9672 /label=polya misc_feature 9632..9672 /label=BBa_B0012 terminator 9632..9672 /label=BBa_B0012 misc_feature 8804..9672 /label=BBa_J06702 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc aaagaggaga aaatgaactc gcctgtaaaa gagatacctg atgtattaaa 1261 aaaccaatgt ggttttaatt gcctgacaga tattagccac agctctttta acgaatttca 1321 ccaacaagta tctgctcacc tctcttggtc cgaagcacac gacttatatc atgatgccca 1381 acaggcccaa aaggataatc ggctatatga agcccgtatt cttaagcgcg cgaatcctca 1441 attacaaaat gccgtacatc ttgccatcat cgcccctaat gtcgaactga taggctataa 1501 cagccaattt agcggtagag ccagtcaata tgtcgcaccg ggtaccgttt cctccatgtt 1561 ctcccccgcc gcttatttga ctgagcttta tcgtgaggct cgcaatttac acgccagcaa 1621 ctccgtttat tatctggata cccgccgccc agatctcaaa tcaatggcgc tcagccaaca 1681 aaatatggat accgaacttt ccacgctctc gttatccaat gagctattgt tggaaagtat 1741 taaaactgag tctaagctgg ataactatac taaagtgatg gaaatgctct ccactttccg 1801 tccttccggc gcgacgcctt atcacgatgc ttatgaaaat gtgcgtgaag ttatccagct 1861 acaagatcct gggcttgagc aattaaatgc atcaccggca attgccgggc taatgcatca 1921 agcctcccta ttgggtatta acgcctcaat ctcacctgag ttgtttaata tcctgacgga 1981 ggagattacc gaaggtaatg ctgaggaact ttatgagaaa aattttggta atatcgaacc 2041 ggcctcactg gctatgccgg aatatcttaa acgttattac aatttaagtg atgaagaact 2101 tagccagttt attggtaaag ccagcaattt tggtcaacag gaatatagtc ataaccagct 2161 tattactcca gtagttaaca gcagtgatgg cacggttaag gtatatcgga ttacccgcga 2221 atatacaaca aatgcctatc aagtggacgt ggaactgttt cccttcggtg gtgaaaatta 2281 tcggttagat tataaattca aagatttttg gaatgccact tatttatcca tcaagttaaa 2341 tgacaaaaga gagcttattc gaactgaagg cgctcctcag gtcaacatag aatactctgc 2401 aaatatcaca ttaagtacca ctgatatcag tcaacctttt gaaattggcc tgacacgagt 2461 acttcctaat actaattacg cctatgccgc cgcaaaattt accgttgagg aatataacca 2521 atactctttc ctgctaaaac tcaataaggc gattcgtcta tcccgagcaa cagaattgtc 2581 acccacgatt ctggaaggta ttgtgcgcag tgttaatcag caactggata tcaacacaga 2641 agtattaggt aaagtttttc tgactaaata ttatatgcag cgttatgcta ttgatgctga 2701 aactgcccta atactctgcc atgcgcttat ttcacaacgt tcatataata atcaaactag 2761 ccagtttgat cgcctgttta atacgccatt actgaacggc caatattttt ctaccggaga 2821 tgaagagatc gatttaaatc caggtagtaa cggtgatttt cgcaaaacca tacttaagag 2881 agcatttaat atcgatgatg tctctctcta ccgcctgctc aaaattactg accataataa 2941 taaagacgga aagattaaaa ataacctaaa ggatctttcc aatttatata ttggaaaatt 3001 actggcagaa atccatcaat taaccattga tgagctggat ttattattgg ttgctgtggg 3061 tgaaggagaa actaatttat ccgctatcag tgataagcaa ttaactactc tgatcagaaa 3121 actcaatacc attaccggct ggttacatat acagaagtgg agcgtgttcc agttgtttat 3181 tatgacctcc accagctata acaaaacgct aacgcctgaa attaagaatc tgctagatac 3241 cgtttaccac ggtttacaag gttttgacaa agacaaagca gatttactac atgttatggc 3301 accctatata gcggctacct tgcaattatc atcggaaaat gtcgctcatt cagtgctgct 3361 ttgggcagac aagttaaagc ccggcgacgg cgcaatgaca gcagaaaaat tctgggactg 3421 gttgaatact cagtatacgc cgggtttatc ggaagcagta gaaatgcggg agcatatcgt 3481 tcagtattgt caagctctgg cacaattgga aatggtttac cattccaccg gtattaacga 3541 aaacgccttc cgcctgtttg taacaaaacc agagatgttt ggcacttcta ttggggcagc 3601 ccctacgcat gatgcgcttt cattgatcat gctgacgcgt tttgcggatt gggttaacgc 3661 gctaggcgaa aaagcctcct ccgtattagc ggcattagaa gctaatactt taacggcaga 3721 acaactggct gacgctatga atcttgatgc taatttgctg ttgcaagcca gtactcaagc 3781 acaaaatcat caacatcttc ctccagtaac acaagaaaat gctttctcct gttggacatc 3841 gatcaatacc atcctgcaat gggttaatgt tgcacaacaa ttgaatgtcg ccccacaggg 3901 cgtttctgct ttagtcggac tggattatat tcaatctaag caaaaaaccc caacctatac 3961 ccagtgggaa aacgcggcgg gaatattaac cgccggatta aattcacaac aagcggatac 4021 attacacgct tttctggatg aatcccgcag tgccgcatta agcacctact atatccgtca 4081 agtcgccaaa ccagcggcgg ccattaaaag ccgtgatgac ctgtatcaat acttgctgat 4141 tgataatcag gtttccgctg caatcaaaac cacccgaatc gccgaagcta ttgccagcat 4201 tcaactgtac gtcaatcggg cgctggaaaa tgtggaagaa aatgccgatt cgggggttat 4261 cagccgccaa ttctttatcg actgggacaa atataataaa cgctacagca cttgggctgg 4321 tgtttctcaa ttagtttact acccggaaaa ctatattgat ccgaccatac gcatcggaca 4381 aaccaaaatg atggacgcat tactgcaatc cgtcagccaa agccaattaa ataccgatac 4441 ggtcgaagac gcctttatgt cttatctgac gtcgtttgaa caggtagcta atcttaaagt 4501 tattagtgca tatcacgata atattaataa cgatcaaggg ctgacctatt ttatcggact 4561 taatgaaact gatgccggtg aatactattg gcgtagtgtc gatcacagta aattcagcga 4621 cggtaaattc gcggctaacg cttggagtga gtggcacaaa attgattgcc caattaatcc 4681 ttaccgaagc actatccgcc ctgtgatgta caaatcccgc ttgtatctgc tctggttgga 4741 acaaaaggag atcaccaaac agacaggaaa tagtaaagat ggctatcaaa ccgagacgga 4801 ttatcgttat gaactgaaac tggcgcatat ccgttatgac ggcacttgga atacgccaat 4861 cacctttgat gtcaatgaaa aaatatccga gctaaaactg gtaaaaaata aagcgcccgg 4921 gctctattgt gccggttatc agggtgaaga tacgttgctg gtgatgtttt ataaccaaca 4981 agatacacta gatagttata aaactgctgc aatgcaaggg ctatatatct ttgccgacat 5041 ggcatccaaa gatatgacct taggacagag caatgtttat cgggataaca gttataaaca 5101 atttgatact aatagtgtca gaagagtgaa taaccgctac gcagagaatt atgaaatccc 5161 ttcatcggta aatagcaata acagttatgg caggggagaa tataacctca gtatggtgca 5221 tggcggagat atcccaatta tccgttacca agcctcatca agtgatttaa aaatccatat 5281 ctcacctaag ttaagaatta cctatgaagg agctgatgag cgaactcgta atcagtgcaa 5341 cctgataaaa aaatatggca aattgggtga taaatttatt atctatacca atctgagtgt 5401 caatacacaa tggacatcaa ataaaaagtt aatatacccg gtttatcaat atagaggaaa 5461 caccactaat aatagtcagg gtagattact gttttatcgg gataaccaaa ttggtaaagt 5521 aagtgtcttt cttcctaatt ggggacaaag cctatattca gaagttacca atagcaataa 5581 tggctatatt atagacactt ctaatcaaga acctttattt aaacaatatc tctatatgga 5641 ggacaaaaat aatggattcc gtattattgc tactgatgtc tataacgcag tagagatcaa 5701 cactgcaatt tccccggcaa aaattcaggt aacagtaaaa gccggtagca aagagaaaac 5761 ttttaccgcg gataaagatg tttccgttca gccatcacct agttttgatg aaatgtatta 5821 tcaatttaat accctcgaaa tagagggtac tggtttgaat tttaataata acttagccag 5881 tattgatgtc acctttactg cattggcgga ggatgggcgt aaactgggtt atgaaagttt 5941 cagtattcct gttactcgca aggtgagtac cgataatgct ctgaccctgc accataatga 6001 aaatggtgcg caatatatgc aatggcaagc ctatcgtact cgcctgaata ccctatttgc 6061 ccgccagttg gttgcacgag ccaccaccgg aatcgataca attctgagta tggaaactca 6121 gaatatccaa gagcctgttt tatggagtga taattttact gcaacattac caatatacaa 6181 ctcaagtctt catggcaaca gcaaaaatat caaaattatg ttaggaaaca gtgaaaaaca 6241 acctgatcaa gcaaatgtca tttatcaggg tgagctaaca gataaaaatc aattaattaa 6301 tctctctgaa acttatatat ccataaaaga gcggagtaac aatacaaaac cagtggataa 6361 taaagggaac gatagctgga tttatttatt tgtaaaatat tctactaatc caataaaaat 6421 tggcgagttt tggtttaaaa atggtacgaa ttttcaaggt tattatccca aaggtaatga 6481 aaaaaaccgt gatcctattt tcatagaaaa tcaaaaaact gaaccaatgg atttcagcgg 6541 cgctaacagt ctttatttct gggaactgtt ctactatacc ccgatgctgg ttgctcaacg 6601 tttgttgcat gaacaaaact ttgatgaagc caaccgctgg ctgaaatatg tctggagtcc 6661 atccggttat attgttcacg gccagattca gaactaccag tggaatgttc gcccgttact 6721 ggaagacacc agttggaaca gtgatccttt agattccgtc gatcctgacg cggtagcaca 6781 acacgatcca atgcactaca aagtctcaac ctttatgcgt actctcgatc tgttgatagc 6841 acgcggcgat caggcttacc gccaactgga gcgggatacg cttaacgaag cgaagatgtg 6901 gtatatgcaa gcgctgcatc tattgggcga caaaccttac ctaccgctga gtacgacatg 6961 gaataatcct cgactagaca gagccgcaga tattactact caaaatgctc acgccagctc 7021 aatagttgct ctgcggcaga gtacaccagc acctttagca ttgcgcagcg ccaataccct 7081 gaccgatctc ttcctgccac aaattaatga agtgatgatg aactactggc agacattagc 7141 tcagagagta tacaacctgc gtcataacct ctctatcgac ggtcaaccgc tatatctgcc 7201 aatctatgcc acaccggcag atccaaaagc gttactcagc gccgccgttg ccacttctca 7261 aagtggaggc aaactaccag agtcatttat gtccctgtgg cgcttcccgc acatgctgga 7321 aaatgcacgc ggcatggtta gccaactcat ccagttcggc tccacgttac aaaatattat 7381 cgagcgtcag gacgcggaag cactcaatgc gttattacaa aatcaggccg cggagctgat 7441 attgactaac ctgagcattc aggacaaaac cattgaagaa ttggatgtcg agaaaaccgt 7501 gctggaaaaa tccaaagcgg gagcccaatc gcgctttgat agctatagca agttgtatga 7561 tgaaaacatc aacgccggtg aaaaccaagc tatgatgcta cgagcgtccg cagccgggct 7621 caccacggca gttcaagcat cccgtctggc tggcgcagcg gctgatttgg tgcctaacat 7681 cttcggcttc gccggcggtg gtagtcgctg gggagctatc gctgaggcaa caggttatgt 7741 gatggaattt tccgctaatg ttatgaatac cgaagcggat aaaattagcc aatccgaaac 7801 ctaccgtcgt cgccgtcagg agtgggagat ccaacgtaat aatgccgaag cagaactgaa 7861 acaactcgat tcccaactta aatcactggt agtacgccgt gaagccgccg tattgcaaaa 7921 aaccagcctg aaaacccagc aagaacagac ccaatctcag ctggccttcc tgcaacgtaa 7981 gttcagcaat caagcgttgt acaactggct acgtggccga ttggcggcga tttacttcca 8041 attctacgat ttggctgtcg cgcgttgttt aatggcagaa caggcttacc gttgggaaat 8101 caatgatgcc tctgcaagtt tcattaaacc gggtgcctgg caaggaacct atgccggtct 8161 gcttgcaggt gaaaccttga tgctaagtct gacacaaatg gaagatgccc atctgaaacg 8221 cgataaacgt gcactagaag tggaacgcac ggtgtcgcta gccgaagttt atgctggatt 8281 gccacaagat aaaggtccat tctccctggc tcaggaaatt gagaagctgg tgagtaaagg 8341 ctcaggcagt gccggcagtg acaataataa tttggcattt ggtgccggta cggacactaa 8401 aacctctttg caggcatcca tttcattagc tgatttgaaa attcgtgagg attacccgac 8461 atctcttggc aaaattcgac gtatcaaaca gatcagtgtc accctgcccg cgctactggg 8521 gccatatcag gatgtgcagg caatattgtc ttacggtgat aaaaccggat tagctaacgg 8581 ctgcgaagcg ctagcagttt ctcacggtat gaatgacagc ggccaattcc agctcgattt 8641 caacgatggc aaattcctgc cattcgaagg catcgccatt gatcaaggca cgctgacact 8701 gagtttccca aatgcatcca tgccgaagaa aggtaaacaa gccactatgc taaaaaccct 8761 gaacgatatc attttgcata ttcgctacac cattaaataa taaaaagagg agaaatacta 8821 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 8881 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 8941 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 9001 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 9061 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 9121 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga 9181 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 9241 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 9301 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 9361 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 9421 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 9481 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aataatacta 9541 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 9601 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 9661 tctgcgttta ta //