BBa_K1669001 1 BBa_K1669001 CtfA + His-tag 2015-09-11T11:00:00Z 2015-09-12T01:47:27Z Sequence retrieved from GenBank and codon optimized for expression in E. coli. This part includes the CtfA gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is one of the two polipeptide chains forming the CtfAB (CoA-transferase) enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyrate to butyril-CoA. The sequence contains a C-terminal His-tag for purification of the protein using the mmobilized-metal affinity chromatography and detected with the anti-His antibodyes. false false _2087_ 27546 27546 9 false Codon optimization for expression in E. coli. Added C-terminal His-tag. false Marina Klemencic annotation2452401 1 CtfA range2452401 1 1 649 annotation2452399 1 double stop codon range2452399 1 669 673 annotation2452400 1 His-tag range2452400 1 650 668 annotation2452398 1 ATG range2452398 1 1 3 BBa_K1669001_sequence 1 atgaatagcaaaattattcgtttcgaaaacctgcgtagctttttcaaggatgggatgaccattatgatcggggggttcttaaattgtgggacaccaaccaaactgattgattttctcgtgaacctgaatattaaaaacttgacaattatcagcaacgacacctgctaccctaacaccggtatcggcaaactgatctcaaataaccaggtgaaaaaattgattgcctcctacatcggctccaatccggatactggcaaaaaactgttcaataacgaacttgaggttgaactgagcccccaaggtacccttgtggaacgtattcgtgcggggggttccggtttaggtggtgttttaacgaaaacgggtttgggaacactgatcgaaaaaggtaagaaaaagatcagtatcaatgggaccgaataccttttagaattgccactgactgccgatgttgcactcattaagggcagcatcgtggatgaagcaggcaatacattctataaaggcactaccaaaaactttaacccctatatggctatggccgcaaaaaccgtgattgttgaagccgagaatctcgtgtcttgtgaaaaactggaaaaagaaaaggccatgaccccgggtgtcttgattaattacatcgtaaaagagccggcacatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z