BBa_K1669004 1 BBa_K1669004 BdhB + His-tag 2015-09-12T11:00:00Z 2015-09-13T02:00:27Z Sequence retrieved from GenBank and codon optimized for expression in E. coli This part includes the BdhB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is the gene coding the butanol dehydrogenase enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyryl-CoA to butanol. The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes. false false _2087_ 26210 26210 9 false C-terminal His-tag. false Nina Jerala annotation2453297 1 double stop codon range2453297 1 1190 1194 annotation2453295 1 BdhB range2453295 1 1 1170 annotation2453294 1 ATG range2453294 1 1 3 annotation2453296 1 His-tag range2453296 1 1171 1189 BBa_K1669004_sequence 1 atggtggatttcgaatattcgattcccacacgcatctttttcggcaaggacaaaattaatgtgctcgggcgggagttgaaaaaatacggctccaaggtcctgatcgtgtacggcggtggctcgatcaagcgcaatggcatttatgataaggctgtaagcattctggaaaaaaactctatcaagttctatgaactggcgggtgtcgagccaaacccgcgtgttactaccgtcgaaaaaggtgtaaaaatctgccgtgaaaatggcgtagaagtggtgcttgcgattggtggcggctctgcgattgattgcgcaaaagtgattgcagcagcgtgtgaatatgatggtaatccgtgggacattgtgctggatggtagcaagattaaacgtgtactgcctatcgcgtcaattctgaccatcgcggccacaggctcggagatggatacatgggcagtgattaataacatggacaccaatgaaaagttaattgccgcacatccagacatggccccgaaattttcgattctggacccgacctatacctataccgtgcccaccaaccagaccgccgccggaaccgctgatattatgtctcatattttcgaagtatatttttctaatacgaaaaccgcctatttacaagaccgtatggccgaagcactgctgcgcacgtgcatcaaatatggcggaatcgcgctggagaaacctgatgactacgaggctcgtgccaaccttatgtgggctagttcactcgccattaatggtttgctcacgtacggtaaggacaccaattggtccgtacacctgatggagcatgaattaagtgcatactacgacattacgcatggcgtgggtctggcaattctcacacctaactggatggaatacattctgaacaacgacacggtgtacaagtttgtggaatatggggttaatgtttggggtattgataaagagaaaaatcactacgatattgcacaccaggcgattcagaagacccgcgattattttgtgaacgtcctcggtctccccagccgtttgcgcgatgtcggcattgaagaagaaaagctggacatcatggctaaagaaagcgtaaaactgaccggcggtactattggtaacctgcgtcccgttaatgcgagcgaagtgctgcaaattttcaagaaaagtgtacatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z