BBa_K1669004
1
BBa_K1669004
BdhB + His-tag
2015-09-12T11:00:00Z
2015-09-13T02:00:27Z
Sequence retrieved from GenBank and codon optimized for expression in E. coli
This part includes the BdhB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli.
This is the gene coding the butanol dehydrogenase enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyryl-CoA to butanol.
The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.
false
false
_2087_
26210
26210
9
false
C-terminal His-tag.
false
Nina Jerala
annotation2453297
1
double stop codon
range2453297
1
1190
1194
annotation2453295
1
BdhB
range2453295
1
1
1170
annotation2453294
1
ATG
range2453294
1
1
3
annotation2453296
1
His-tag
range2453296
1
1171
1189
BBa_K1669004_sequence
1
atggtggatttcgaatattcgattcccacacgcatctttttcggcaaggacaaaattaatgtgctcgggcgggagttgaaaaaatacggctccaaggtcctgatcgtgtacggcggtggctcgatcaagcgcaatggcatttatgataaggctgtaagcattctggaaaaaaactctatcaagttctatgaactggcgggtgtcgagccaaacccgcgtgttactaccgtcgaaaaaggtgtaaaaatctgccgtgaaaatggcgtagaagtggtgcttgcgattggtggcggctctgcgattgattgcgcaaaagtgattgcagcagcgtgtgaatatgatggtaatccgtgggacattgtgctggatggtagcaagattaaacgtgtactgcctatcgcgtcaattctgaccatcgcggccacaggctcggagatggatacatgggcagtgattaataacatggacaccaatgaaaagttaattgccgcacatccagacatggccccgaaattttcgattctggacccgacctatacctataccgtgcccaccaaccagaccgccgccggaaccgctgatattatgtctcatattttcgaagtatatttttctaatacgaaaaccgcctatttacaagaccgtatggccgaagcactgctgcgcacgtgcatcaaatatggcggaatcgcgctggagaaacctgatgactacgaggctcgtgccaaccttatgtgggctagttcactcgccattaatggtttgctcacgtacggtaaggacaccaattggtccgtacacctgatggagcatgaattaagtgcatactacgacattacgcatggcgtgggtctggcaattctcacacctaactggatggaatacattctgaacaacgacacggtgtacaagtttgtggaatatggggttaatgtttggggtattgataaagagaaaaatcactacgatattgcacaccaggcgattcagaagacccgcgattattttgtgaacgtcctcggtctccccagccgtttgcgcgatgtcggcattgaagaagaaaagctggacatcatggctaaagaaagcgtaaaactgaccggcggtactattggtaacctgcgtcccgttaatgcgagcgaagtgctgcaaattttcaagaaaagtgtacatcaccatcatcaccattaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z