LOCUS       BBa_K1669005             790 bp    DNA     linear       13-May-2024
DEFINITION  CtfB with double terminator
ACCESSION   BBa_K1669005
VERSION     BBa_K1669005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=ATG
     CDS             1..673
                     /label=CtfB
     misc_feature    674..682
                     /label=His-tag
     misc_feature    683..687
                     /label=double stop codon
     CDS             1..687
                     /label=BBa_K1669003
     stem_loop       703..722
                     /label=T7 TE
     misc_feature    729..729
                     /label=stop
     polyA_site      723..736
                     /label=polya
     misc_feature    696..736
                     /label=BBa_B0012
     terminator      696..736
                     /label=BBa_B0012
     stem_loop       757..779
                     /label=stem_loop
     misc_feature    745..790
                     /label=BBa_B0011
     terminator      745..790
                     /label=BBa_B0011
     terminator      696..790
                     /label=BBa_B0014
     terminator      696..790
                     /label=BBa_K823017
ORIGIN
        1 atgattaatg ataaaaatct tgccaaggaa atcatcgcga aacgcgtggc gcgggaactt
       61 aaaaatggcc agctggtgaa cctgggcgtt ggcctgccga ccatggtagc ggattatatc
      121 ccgaaaaatt ttaaaatcac ctttcagagc gagaacggga ttgtgggtat gggcgcatca
      181 ccaaaaatta atgaagccga taaagatgtt gtgaatgcgg gtggcgatta caccacggtg
      241 ttgcctgatg gtacgttctt cgatagcagc gtgtcttttt cgcttatccg cggtggccac
      301 gtagatgtaa cggtgctcgg tgcgttacag gtggacgaaa agggtaatat cgcgaactgg
      361 atcgtccctg gtaagatgct gtccggaatg ggtggtgcaa tggatcttgt caacggcgct
      421 aagaaagtta ttattgccat gcgtcacacg aacaagggac agccgaaaat ccttaaaaag
      481 tgcaccttgc cacttacggc gaaatcacaa gcaaatctca ttgtgactga actgggcgtg
      541 atcgaagtga ttaatgacgg cctgttgctg acagaaatca acaaaaacac gaccatcgat
      601 gaaattcgta gtctgactgc cgccgatctg cttattagca acgaactgcg cccgatggcg
      661 gtgcatcacc atcatcacca ttaataatac tagagtcaca ctggctcacc ttcgggtggg
      721 cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta atccggcttt
      781 tttattattt
//