LOCUS       BBa_K1669006            1297 bp    DNA     linear       14-May-2024
DEFINITION  BdhB with double terminator
ACCESSION   BBa_K1669006
VERSION     BBa_K1669006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=ATG
     CDS             1..1170
                     /label=BdhB
     misc_feature    1171..1189
                     /label=His-tag
     misc_feature    1190..1194
                     /label=double stop codon
     CDS             1..1194
                     /label=BBa_K1669004
     stem_loop       1210..1229
                     /label=T7 TE
     misc_feature    1236..1236
                     /label=stop
     polyA_site      1230..1243
                     /label=polya
     misc_feature    1203..1243
                     /label=BBa_B0012
     terminator      1203..1243
                     /label=BBa_B0012
     stem_loop       1264..1286
                     /label=stem_loop
     misc_feature    1252..1297
                     /label=BBa_B0011
     terminator      1252..1297
                     /label=BBa_B0011
     terminator      1203..1297
                     /label=BBa_B0014
     terminator      1203..1297
                     /label=BBa_K823017
ORIGIN
        1 atggtggatt tcgaatattc gattcccaca cgcatctttt tcggcaagga caaaattaat
       61 gtgctcgggc gggagttgaa aaaatacggc tccaaggtcc tgatcgtgta cggcggtggc
      121 tcgatcaagc gcaatggcat ttatgataag gctgtaagca ttctggaaaa aaactctatc
      181 aagttctatg aactggcggg tgtcgagcca aacccgcgtg ttactaccgt cgaaaaaggt
      241 gtaaaaatct gccgtgaaaa tggcgtagaa gtggtgcttg cgattggtgg cggctctgcg
      301 attgattgcg caaaagtgat tgcagcagcg tgtgaatatg atggtaatcc gtgggacatt
      361 gtgctggatg gtagcaagat taaacgtgta ctgcctatcg cgtcaattct gaccatcgcg
      421 gccacaggct cggagatgga tacatgggca gtgattaata acatggacac caatgaaaag
      481 ttaattgccg cacatccaga catggccccg aaattttcga ttctggaccc gacctatacc
      541 tataccgtgc ccaccaacca gaccgccgcc ggaaccgctg atattatgtc tcatattttc
      601 gaagtatatt tttctaatac gaaaaccgcc tatttacaag accgtatggc cgaagcactg
      661 ctgcgcacgt gcatcaaata tggcggaatc gcgctggaga aacctgatga ctacgaggct
      721 cgtgccaacc ttatgtgggc tagttcactc gccattaatg gtttgctcac gtacggtaag
      781 gacaccaatt ggtccgtaca cctgatggag catgaattaa gtgcatacta cgacattacg
      841 catggcgtgg gtctggcaat tctcacacct aactggatgg aatacattct gaacaacgac
      901 acggtgtaca agtttgtgga atatggggtt aatgtttggg gtattgataa agagaaaaat
      961 cactacgata ttgcacacca ggcgattcag aagacccgcg attattttgt gaacgtcctc
     1021 ggtctcccca gccgtttgcg cgatgtcggc attgaagaag aaaagctgga catcatggct
     1081 aaagaaagcg taaaactgac cggcggtact attggtaacc tgcgtcccgt taatgcgagc
     1141 gaagtgctgc aaattttcaa gaaaagtgta catcaccatc atcaccatta ataatactag
     1201 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
     1261 aaaaagccag attattaatc cggctttttt attattt
//