LOCUS       BBa_K1669007             842 bp    DNA     linear       14-May-2024
DEFINITION  CtfA with strong promotor, strong RBS and double terminator
ACCESSION   BBa_K1669007
VERSION     BBa_K1669007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     misc_feature    62..64
                     /label=ATG
     misc_feature    730..734
                     /label=double stop codon
     misc_feature    711..729
                     /label=His-tag
     CDS             62..710
                     /label=CtfA
     CDS             62..739
                     /label=BBa_K1669001
     stem_loop       755..774
                     /label=T7 TE
     misc_feature    781..781
                     /label=stop
     polyA_site      775..788
                     /label=polya
     misc_feature    748..788
                     /label=BBa_B0012
     terminator      748..788
                     /label=BBa_B0012
     stem_loop       809..831
                     /label=stem_loop
     misc_feature    797..842
                     /label=BBa_B0011
     terminator      797..842
                     /label=BBa_B0011
     terminator      748..842
                     /label=BBa_B0014
     terminator      748..842
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgaatagc aaaattattc gtttcgaaaa cctgcgtagc tttttcaagg atgggatgac
      121 cattatgatc ggggggttct taaattgtgg gacaccaacc aaactgattg attttctcgt
      181 gaacctgaat attaaaaact tgacaattat cagcaacgac acctgctacc ctaacaccgg
      241 tatcggcaaa ctgatctcaa ataaccaggt gaaaaaattg attgcctcct acatcggctc
      301 caatccggat actggcaaaa aactgttcaa taacgaactt gaggttgaac tgagccccca
      361 aggtaccctt gtggaacgta ttcgtgcggg gggttccggt ttaggtggtg ttttaacgaa
      421 aacgggtttg ggaacactga tcgaaaaagg taagaaaaag atcagtatca atgggaccga
      481 atacctttta gaattgccac tgactgccga tgttgcactc attaagggca gcatcgtgga
      541 tgaagcaggc aatacattct ataaaggcac taccaaaaac tttaacccct atatggctat
      601 ggccgcaaaa accgtgattg ttgaagccga gaatctcgtg tcttgtgaaa aactggaaaa
      661 agaaaaggcc atgaccccgg gtgtcttgat taattacatc gtaaaagagc cggcacatca
      721 ccatcatcac cattaataat actagagtca cactggctca ccttcgggtg ggcctttctg
      781 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
      841 tt
//