BBa_K1670003

BBa_K1670003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1670003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Christoph Schilling

Date created: 2015-08-24 11:00:00

Date modified: 2015-08-27 04:33:50

aidA-Promoter + mRFP

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1670003_sequence (Version 1)

Description

BBa_K167003 (P_aidA_mRFP) contains the CepR/I regulated aidA promoter. The CepR binding box is located 44 bp upstream of the predicted transcription start. The part also encodes for a mRFP fluorescent reporter, based on BBa_K1362461. Two silent mutations (T213A, T435A) were introduced to delete two HindIII recognition sites. mRFP can be exchanged using an XhoI restriction site directly upstream of the start codon and the biobrick suffix.

Notes

strong ribosome binding site
mRFP based on BBa_K1362461(2 silent mutations at 213 and 435 T->A to delete HindIII recognition sites)
CepR-binding box in promoter

Source

Genome Sequence from Burkholderia cenocepacia GenBank: HG938372.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
aidA_Promoter	1,173	feature/promoter, promoter
cepR-Box	8,23	non_covalent_binding_site, feature/binding
RBS	174,197	ribosome_entry_site, feature/rbs
mRFP	198,872	feature/cds, CDS
STOP	873,878	feature/stop, stop_codon

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1670003/1

Member of these Collections

• iGEM Parts Registry