BBa_K1670003
1
BBa_K1670003
aidA-Promoter + mRFP
2015-08-24T11:00:00Z
2015-08-27T04:33:50Z
Genome Sequence from <i> Burkholderia cenocepacia </i> GenBank: HG938372.1
BBa_K167003 (P<sub>aidA</sub>_mRFP) contains the CepR/I regulated aidA promoter. The CepR binding box is located 44 bp upstream of the predicted transcription start. The part also encodes for a mRFP fluorescent reporter, based on BBa_K1362461. Two silent mutations (T213A, T435A) were introduced to delete two HindIII recognition sites. mRFP can be exchanged using an XhoI restriction site directly upstream of the start codon and the biobrick suffix.
false
false
_2088_
24905
24905
9
false
strong ribosome binding site
mRFP based on BBa_K1362461(2 silent mutations at 213 and 435 T->A to delete HindIII recognition sites)
CepR-binding box in promoter
false
Christoph Schilling
annotation2438513
1
RBS
range2438513
1
174
197
annotation2438511
1
aidA_Promoter
range2438511
1
1
173
annotation2438515
1
STOP
range2438515
1
873
878
annotation2438512
1
cepR-Box
range2438512
1
8
23
annotation2438514
1
mRFP
range2438514
1
198
872
BBa_K1670003_sequence
1
tcccgacctgtttacttttacagcttccatgattaaccaaacggactaatctgcattagccgtcgcctccgcacgtgccgataccggcgcaaacccttgcctcacaaggcgatggcggctgcacgaagcaattcgcgcaacgatgattcccattacttcaaacgggagcttctaatattaactaagggggtctcgagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcatacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcatccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z