LOCUS       BBa_K1673214             676 bp    DNA     linear       12-May-2024
DEFINITION  Mutation Optimized T7 Endonuclease with IPTG Promoter
ACCESSION   BBa_K1673214
VERSION     BBa_K1673214.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..676
                     /label=BBa_K1673204
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctggttatgg
      241 tgctaaaggt ataagaaaag ttggtgcttt tagaagtggt cttgaagata aagttagtaa
      301 acaacttgaa agtaaaggta taaaatttga atatgaagaa tggaaagttc cttatgttat
      361 acctgctagt aatcatactt atactcctga ttttcttctt cctaatggta tatttgttga
      421 aactaaaggt ctttgggaaa gtgatgatag aaaaaaacat cttcttataa gagaacaaca
      481 tcctgaactt gatataagaa tagtttttag tagtagtaga actaaacttt ataaaggtag
      541 tcctactagc tatggtgaat tttgtgaaaa acatggtata aaatttgctg ataaacttat
      601 acctgctgaa tggataaaag aacctaaaaa agaagttcct tttgatagac ttaaaagaaa
      661 aggtggtaaa aaatga
//