LOCUS       BBa_K1675015            2841 bp    DNA     linear       14-May-2024
DEFINITION  J23119+B0034+NhaA+J23119+B0034+NhaB
ACCESSION   BBa_K1675015
VERSION     BBa_K1675015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..66
                     /label=GTG Start Codon
     CDS             64..1230
                     /label=NhaA Coding Region
     misc_feature    1228..1230
                     /label=TGA Stop Codon
     CDS             64..1230
                     /label=BBa_K1675000
     promoter        1239..1273
                     /label=BBa_J23119
     misc_feature    1286..1289
                     /label=conserved
     RBS             1282..1293
                     /label=BBa_B0034
     CDS             1300..2841
                     /label=NhaB Coding Region
     misc_feature    1300..1302
                     /label=ATG start codon
     misc_feature    2839..2841
                     /label=TGA stop codon
     CDS             1300..2841
                     /label=BBa_K1675001
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gaggtgaaac atctgcatcg attctttagc agtgatgcct cgggaggcat tattcttatc
      121 attgccgcta tcctggcgat gattatggcc aacagcggcg caaccagtgg atggtatcac
      181 gactttctgg agacgccggt tcagctccgg gttggttcac tcgaaatcaa caaaaacatg
      241 ctgttatgga taaatgacgc gctgatggcg gtatttttcc tgttagtcgg tctggaagtt
      301 aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc aggccgcatt tccagttatc
      361 gccgctattg gtgggatgat tgtgccggca ttactctatc tggcttttaa ctatgccgat
      421 ccgattaccc gcgaagggtg ggcgatcccg gcggctactg acattgcttt tgcacttggt
      481 gtactggcgc tgttgggaag tcgtgttccg ttagcgctga agatcttttt gatggctctg
      541 gctattatcg acgatcttgg ggccatcatt atcatcgcat tgttctacac taatgactta
      601 tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg tactcgcggt attgaatctg
      661 tgtggtgcac gccgcacggg cgtctatatt cttgttggcg tggtgttgtg gactgcggtg
      721 ttgaaatcgg gggttcacgc aactctggcg ggggtaattg tcggcttctt tattcctttg
      781 aaagagaagc atgggcgttc tccagcgaag cgactggagc atgtgttgca cccgtgggtg
      841 gcgtatctga ttttgccgct gtttgcattt gctaatgctg gcgtttcact gcaaggcgtc
      901 acgctggatg gcttgacctc cattctgcca ttggggatca tcgctggctt gctgattggc
      961 aaaccgctgg ggattagtct gttctgctgg ttggcgctgc gtttgaaact ggcgcatctg
     1021 cctgagggaa cgacttatca gcaaattatg gtggtgggga tcctgtgcgg tatcggtttt
     1081 actatgtcta tctttattgc cagcctggcc tttggtagcg tagatccaga actgattaac
     1141 tgggcgaaac tcggtatcct ggtcggttct atctcttcgg cggtaattgg atacagctgg
     1201 ttacgcgttc gtttgcgtcc atcagtttga tactagagtt gacagctagc tcagtcctag
     1261 gtataatgct agctactaga gaaagaggag aaatactaga tggagatctc ctggggccgc
     1321 gcgctatggc gcaatttttt gggccagtcc cccgactggt acaaactcgc cctcatcatt
     1381 ttcttaatcg taaacccgtt aattttcctc atcagccctt tcgtcgcggg ctggttgctg
     1441 gtagcggaat ttattttcac tctggcgatg gccctgaaat gctacccgct gctccccggt
     1501 ggtctgttgg ctatcgaagc ggtattcatc ggcatgacca gcgcggaaca cgtccgtgaa
     1561 gaggtggcgg caaatcttga agtcttgctg ttactgatgt ttatggtggc gggtatctat
     1621 tttatgaaac agctattgct gttcatattt acccgtttgc tgttaagcat tcgctccaaa
     1681 atgctgctgt cgctctcttt ttgcgtggcg gcggcgttcc tctccgcgtt cctcgatgcc
     1741 ttaaccgtcg tggcggtggt gatcagcgtt gcagtcggtt tttatggtat ttatcaccgc
     1801 gtagcctctt cccgtactga agataccgac ctgcaagacg atagtcatat cgacaagcat
     1861 tacaaagtgg ttctggaaca gttccgtggc tttctgcgta gcctgatgat gcatgccggt
     1921 gtcggcactg cattaggcgg cgtgatgacc atggtgggcg aaccacagaa cctgatcatc
     1981 gctaaagcgg ctggctggca ttttggcgat ttcttcctgc gcatgtcgcc ggtgaccgtt
     2041 ccggttctga tttgtggcct gttaacctgc ctgctggtag agaagctgcg ttggtttggc
     2101 tacggtgaaa cgctgccgga gaaagtccgc gaagtgttgc aacagtttga cgatcaaagc
     2161 cgccaccagc gtacccgtca ggataaaatc cgtctgattg tccaggcgat tattggcgtc
     2221 tggctggtga ctgcgctggc gttgcatctg gcggaagttg gcttgattgg tttgtcagtc
     2281 attattctgg cgacatcatt gaccggtgtc accgatgaac atgctatcgg taaagccttc
     2341 accgaatctc tgccattcac cgcactgttg acggtgtttt tctcggtagt cgcggtgatt
     2401 atcgaccaac aactgttttc gccaattatt cagtttgtgt tgcaggcatc ggaacatgct
     2461 cagctgtcgc tgttctatat tttcaacggt ctgctgtcat ccatttcgga taacgtcttc
     2521 gtggggacga tttatatcaa cgaagcgaaa gcggcaatgg aaagtggcgc tatcacgttg
     2581 aagcaatacg agctgctggc ggtcgccatt aataccggta ccaatctgcc ctccgtcgct
     2641 acgccgaacg gtcaggctgc gttcctgttc ctgctgacct ctgcactcgc gccattgatt
     2701 cgcctctctt atggccgcat ggtgtggatg gccctgcctt acaccctcgt actgacactc
     2761 gtcggcttgc tctgcgtcga gtttacgctt gcccctgtaa ccgaatggtt tatgcaaatg
     2821 ggctggatag caacgctttg a
//