LOCUS       BBa_K1679019            1690 bp    DNA     linear       17-May-2024
DEFINITION  GFP+Promoter+RNA thermometer (FourU) +mRFP
ACCESSION   BBa_K1679019
VERSION     BBa_K1679019.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     misc_feature    1..875
                     /label=BBa_I13504
     promoter        884..918
                     /label=BBa_J23119
     RBS             973..978
                     /label=rbs
     misc_feature    949..954
                     /label=scar adaptation
     stem_loop       927..944
                     /label=stem_loop
     stem_loop       950..978
                     /label=predicted stem-loop extending to one position 
                     before start codon
     misc_feature    927..978
                     /label=BBa_K115002
     CDS             985..1659
                     /label=mrfp1
     misc_feature    1666..1690
                     /label=Help:Barcodes
     CDS             985..1690
                     /label=BBa_E1010
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag ctagctcagt
      901 cctaggtata atgctagcta ctagagggac aagcaatgct tgccttgaat agtaactttt
      961 gaatagtgat tcaggaggta ctagatggct tcctccgaag acgttatcaa agagttcatg
     1021 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
     1081 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
     1141 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
     1201 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
     1261 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
     1321 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
     1381 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     1441 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     1501 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1561 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1621 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
     1681 tgtagatcgc
//