BBa_K1680018

BBa_K1680018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicolai von K??gelgen

Date created: 2015-09-16 11:00:00

Date modified: 2016-01-26 10:15:36

Cre-Dronpa fusion

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1680018_sequence (Version 1)

Description

Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 9 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.

Notes

Assembled using 3A assembly and RFC25.

Source

gBlocks designed by Team Tuebingen

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
K1680006	1078,1746	feature/cds, CDS
K1680007	22,1050	feature/cds, CDS
K1680001	1,21	CDS, feature/cds
K1680001	1051,1077	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680018/1

Member of these Collections

• //cds
• //cds/reporter/chromoprotein
• //cds/reporter/gfp
• //chassis/eukaryote/yeast
• //function/recombination/cre
• //function/reporter/fluorescence
• iGEM Parts Registry