BBa_K1680020

BBa_K1680020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicolai von Kuegelgen

Date created: 2015-09-16 11:00:00

Date modified: 2016-01-26 10:16:19

Cre-dronpa fusion

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1680020_sequence (Version 1)

Description

Protein coding region for a fusion construct of Cre Recombinase and 145N Dronpa with 15 amino acid linker. Oligomerisation of Dronpa should stop Cre from accessing DNA in the nucleus thereby inhibiting it until light deactivation of dronpa.

Notes

3A assembly using RFC25

Source

gBlock design by Team Tuebingen

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
K1680007	40,1068	feature/cds, CDS
K1680006	1114,1782	feature/cds, CDS
K1680003	1,39	CDS, feature/cds
K1680003	1089,1113	feature/cds, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680020/1

Member of these Collections

• //cds
• //cds/reporter/chromoprotein
• //cds/reporter/gfp
• //chassis/eukaryote/yeast
• //function/recombination/cre
• //function/reporter/fluorescence
• iGEM Parts Registry