BBa_K1680022

BBa_K1680022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1680022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicolai von Kuegelgen
Date created: 2015-09-16 11:00:00
Date modified: 2015-10-27 01:52:38

Dronpa caged Cre with NLS



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1680022_sequence (Version 1)

Description

Protein coding region for a fusion protein of Cre recombinase (BBa_K1680007) that is C- & N-terminal flanked with 145N Dronpa (BBa_K1680006) using a 9 AA linker as spacer (BBa_K1680001). The construct has a N-terminal NLS sequence (BBa_K1680004). In the expressed construct the two dronpa domains should form multimerize leading to an inactive Cre recombinase that is reversibly activatable through dronpa illumination (see Zhou et al 2012).

Notes

3A Assembly using RFC25

Source

gBlock designed by Team Tuebingen

Sequence Annotation Location Component / Role(s)
SV40 NLS
Dronpa
Linker
Cre recombinase
Linker
Dronpa
4,24
31,699
700,726
727,1755
1756,1783
1783,2451
sequence_feature feature/misc
CDS feature/protein
feature/misc sequence_feature
CDS feature/protein
sequence_feature feature/misc
CDS feature/protein
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1680022/1