LOCUS       BBa_K1694044            2100 bp    DNA     linear       10-May-2024
DEFINITION  Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+RFP+J61048
ACCESSION   BBa_K1694044
VERSION     BBa_K1694044.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..501
                     /label=BBa_K1694002
     CDS             510..1244
                     /label=BBa_K1694004
     misc_feature    64..1244
                     /label=BBa_K1694014
     misc_feature    1..1244
                     /label=BBa_K1694024
     RBS             1253..1267
                     /label=RBS-1\Strong
     RBS             1260..1264
                     /label=RBS
     misc_feature    1253..1267
                     /label=BBa_B0030
     RBS             1253..1267
                     /label=BBa_B0030
     CDS             1274..1948
                     /label=mrfp1
     misc_feature    1955..1979
                     /label=Help:Barcodes
     CDS             1274..1979
                     /label=BBa_E1010
     terminator      1988..2100
                     /label=BBa_J61048
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gagaaagcaa ccaagctggt tctgggtgcc gtgattctgg gcagtaccct gttagcaggt
      121 tgttctagca atgccaaaat cgaccaaggc atcaacaaca atggcccgac ccacgaaaac
      181 cagctgggtg ccggtgcctt tggtggttat caggtgaacc cgtacgtggg ctttgaaatg
      241 ggctatgatt ggctgggccg catgccgtac aaaggcagtg tggagaacgg cgcctataaa
      301 gcacagggcg tgcagctgac agcaaaactg ggctacccta ttaccgacga cctggacatc
      361 tacacacgct taggcggcat ggtgtggcgc gccgatacca agagcaacgt gtacggcaag
      421 aaccacgata ccggcgtgag tccggtgttt gccggcggtg tggagtatgc aatcaccccg
      481 gaaattgcca cacgtccatg gtactagagg atattctgct gacccagagc ccggtgatcc
      541 tgagcgtgag tccgggtgaa cgtgtgagct tcagttgccg tgccagccag agcatcggta
      601 ccaacatcca ctggtatcag cagcgcacca acggtagtcc gcgcctgctg atcaaatatg
      661 ccagcgagag catcagcggc atcccgagcc gctttagtgg tagcggcagc ggcaccgatt
      721 ttaccctgag catcaacagc gtggaaagcg aagacatcgc agattattat tgccagcaga
      781 acaataattg gccgaccacc ttcggcgccg gtaccaaact ggaactgaaa ggcggtagca
      841 gccgtagtag cagcagtggt ggtggtggta gtggtggcgg tggccaggtt cagctgaaac
      901 agagcggtcc gggtctggtg cagcctagcc agagcctgag cattacctgc accgtgagcg
      961 gctttagcct gaccaattac ggcgtgcatt gggtgcgtca gagcccgggc aagggtctgg
     1021 aatggctggg tgttatctgg agcggcggca ataccgatta caacaccccg ttcaccagcc
     1081 gcctgagtat taacaaagat aatagcaaaa gccaggtctt ttttaaaatg aatagcttac
     1141 agagcaatga caccgcaatt tattactgcg cccgcgccct gacctactat gattatgagt
     1201 tcgcatactg gggtcagggc accctggtga cagtgagtgc ataatactag agattaaaga
     1261 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
     1321 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
     1381 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
     1441 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
     1501 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
     1561 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
     1621 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
     1681 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
     1741 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
     1801 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
     1861 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
     1921 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
     1981 actagagccg gcttatcggt cagtttcacc tgatttacgt aaaaacccgc ttcggcgggt
     2041 ttttgctttt ggaggggcag aaagatgaat gactgtccac gacgctatac ccaaaagaaa
//