BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1694014 1 BBa_K1694014 OmpA-scFv(Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T09:37:51Z Assemble We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab false false _2112_ 27153 28523 9 false No false CHIH-HSUAN HSU component2457314 1 BBa_K1694004 component2457313 1 BBa_K1694002 annotation2457314 1 BBa_K1694004 range2457314 1 447 1181 annotation2457313 1 BBa_K1694002 range2457313 1 1 438 BBa_J61048 1 BBa_J61048 [rnpB-T1] Terminator 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1694004 1 BBa_K1694004 Single-chain variable fragment (Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T07:29:10Z Artificial synthesis We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab false false _2112_ 28523 28523 9 true No false CHIH-HSUAN HSU BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_K1694002 1 BBa_K1694002 Lpp-OmpA-N 2015-09-14T11:00:00Z 2015-09-18T09:58:58Z Artificial synthesis To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU BBa_K1694044 1 BBa_K1694044 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+RFP+J61048 2015-09-14T11:00:00Z 2015-09-18T10:05:30Z Assembly J false false _2112_ 27160 28523 9 true No false CHIH-HSUAN HSU component2457517 1 BBa_J61048 component2457512 1 BBa_B0030 component2457510 1 BBa_K1694024 component2457516 1 BBa_E1010 annotation2457517 1 BBa_J61048 range2457517 1 1988 2100 annotation2457510 1 BBa_K1694024 range2457510 1 1 1244 annotation2457512 1 BBa_B0030 range2457512 1 1253 1267 annotation2457516 1 BBa_E1010 range2457516 1 1274 1979 BBa_K1694024 1 BBa_K1694024 Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR) 2015-09-14T11:00:00Z 2015-09-18T08:07:32Z Assembly By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Cetuximab) outside the E.coli cell membrane. false false _2112_ 28523 28523 9 false No false CHIH-HSUAN HSU component2457387 1 BBa_K1694014 component2457382 1 BBa_J23101 component2457384 1 BBa_B0034 annotation2457387 1 BBa_K1694014 range2457387 1 64 1244 annotation2457384 1 BBa_B0034 range2457384 1 44 55 annotation2457382 1 BBa_J23101 range2457382 1 1 35 BBa_K1694024_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_K1694014_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_J61048_sequence 1 ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_B0030_sequence 1 attaaagaggagaaa BBa_K1694002_sequence 1 aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg BBa_K1694004_sequence 1 gatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1694044_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagaggatattctgctgacccagagcccggtgatcctgagcgtgagtccgggtgaacgtgtgagcttcagttgccgtgccagccagagcatcggtaccaacatccactggtatcagcagcgcaccaacggtagtccgcgcctgctgatcaaatatgccagcgagagcatcagcggcatcccgagccgctttagtggtagcggcagcggcaccgattttaccctgagcatcaacagcgtggaaagcgaagacatcgcagattattattgccagcagaacaataattggccgaccaccttcggcgccggtaccaaactggaactgaaaggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccaggttcagctgaaacagagcggtccgggtctggtgcagcctagccagagcctgagcattacctgcaccgtgagcggctttagcctgaccaattacggcgtgcattgggtgcgtcagagcccgggcaagggtctggaatggctgggtgttatctggagcggcggcaataccgattacaacaccccgttcaccagccgcctgagtattaacaaagataatagcaaaagccaggtcttttttaaaatgaatagcttacagagcaatgacaccgcaatttattactgcgcccgcgccctgacctactatgattatgagttcgcatactggggtcagggcaccctggtgacagtgagtgcataatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z