BBa_K1694005
1
BBa_K1694005
Single-chain variable fragment (Anti-HER2)
2015-09-14T11:00:00Z
2015-09-18T07:36:18Z
Artificial synthesis
We selected the single chain variable fragments (scFv) of monoclonal antibodies Trastuzumab
false
false
_2112_
28523
28523
9
false
No
false
CHIH-HSUAN HSU
BBa_K1694025
1
BBa_K1694025
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)
2015-09-14T11:00:00Z
2015-09-18T09:01:40Z
Assembly
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and Lpp-OmpA-scFv, we were able to display scFv(Trastuzumab) outside the E.coli cell membrane.
false
false
_2112_
28523
28523
9
false
No
false
CHIH-HSUAN HSU
component2457401
1
BBa_K1694015
component2457398
1
BBa_B0034
component2457396
1
BBa_J23101
annotation2457396
1
BBa_J23101
range2457396
1
1
35
annotation2457398
1
BBa_B0034
range2457398
1
44
55
annotation2457401
1
BBa_K1694015
range2457401
1
64
1089
BBa_K592100
1
BFP
Blue Fluorescent Protein (mTagBFP)
2011-09-19T11:00:00Z
2016-01-25T04:45:27Z
Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.
Released HQ 2013
This part codes for the bright blue fluorescent protein mTagBFP. mTagBFP is a monomeric protein with a narrow fluorescence emission spectrum with a maximum at 456 nm. It has a tyrosine-based chromophore, giving it substantially higher brightness, faster chromophore maturation and higher pH stability than blue fluorescent proteins with a histidine in the chromophore. Subach et. al. started with a red fluorescent protein (TagRed) and converted it to a bright blue monomeric protein. See the article listed in source. The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
false
_763_
4206
10137
9
In stock
true
The sequence has been codon optimized for expression in E coli by DNA 2.0.
false
Erik Gullberg
annotation2135490
1
mTagBFP
range2135490
1
1
699
BBa_J61048
1
BBa_J61048
[rnpB-T1] Terminator
2007-02-20T12:00:00Z
2015-08-31T02:03:00Z
bob
bob
false
false
_95_
0
483
95
In stock
false
bob
true
John Anderson
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K1694015
1
BBa_K1694015
OmpA-scFv(Anti-HER2)
2015-09-14T11:00:00Z
2015-09-18T08:50:13Z
Assemble
We selected the single chain variable fragments (scFv) of monoclonal antibodies Trastuzumab
false
false
_2112_
28523
28523
9
false
No
false
CHIH-HSUAN HSU
component2457346
1
BBa_K1694002
component2457347
1
BBa_K1694005
annotation2457347
1
BBa_K1694005
range2457347
1
445
1026
annotation2457346
1
BBa_K1694002
range2457346
1
1
438
BBa_K1694002
1
BBa_K1694002
Lpp-OmpA-N
2015-09-14T11:00:00Z
2015-09-18T09:58:58Z
Artificial synthesis
To display the antibody outside the E.coli, we used Lipoprotein-Outer membrane protein A (Lpp-OmpA)and added a NcoI restriction side behind OmpA
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
BBa_K1694045
1
BBa_K1694045
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-HER2)+B0030+BFP+J61048
2015-09-14T11:00:00Z
2015-09-18T10:08:35Z
Assembly
F
false
false
_2112_
27160
28523
9
true
No
false
CHIH-HSUAN HSU
component2457561
1
BBa_B0030
component2457564
1
BBa_K592100
component2457565
1
BBa_J61048
component2457559
1
BBa_K1694025
annotation2457564
1
BBa_K592100
range2457564
1
1119
1823
annotation2457561
1
BBa_B0030
range2457561
1
1098
1112
annotation2457559
1
BBa_K1694025
range2457559
1
1
1089
annotation2457565
1
BBa_J61048
range2457565
1
1832
1944
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1694005_sequence
1
atgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt
BBa_K1694015_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt
BBa_J61048_sequence
1
ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_K1694045_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgttactagagattaaagaggagaaatactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1694002_sequence
1
aaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatgg
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K592100_sequence
1
atgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaattaataa
BBa_K1694025_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagagaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtccatggtactagatgacccagtctccgaaattcatgtccacctcagtgggcgatagggtcagcattacctgcaaagccagccaggatgtgaataccgcggtggcctggtatcagcagaaaccagggcagtctcctaaactactgatttactgggcgtccacccgtcataccggcgtccctgatcgcttcaccggcagcggctctggcaccgattataccctgaccattagcagcgtgcaggctgaagatctggcgctttattactgtggcggtagcagccgtagtagcagcagtggtggtggtggtagtggtggcggtggccagcctggctctgaactggtgaaacctggggcttcagtgaaactgtcctgtaaggcgtctggctacaccttcaccagcgattggatgcactgggtgaaacagaggcctggccagggccttgaatggattggcgaaattaatcctagcaacggtcgtaccaactacaatgaaaaattcaaaagcaaagccaccctgaccgtggataaatcctccagcaccgcctacatgcaggtcagcagcctgacctctgaagattctgcggtctattactgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z