LOCUS BBa_K1697002 3928 bp DNA linear 17-May-2024 DEFINITION Toggle switch for gram positive ACCESSION BBa_K1697002 VERSION BBa_K1697002.1 FEATURES Location/Qualifiers misc_feature 51..61 /label=XylR Operator misc_feature 65..75 /label=XylR Operator promoter 13..18 /label=Sigma A -35 promoter 36..41 /label=Sigma A -10 promoter 1..82 /label=BBa_K143014 RBS 83..94 /label=rbs RBS 83..94 /label=BBa_K143021 CDS 95..1174 /label=LacI (Lva-, N-terminal deletion) regulatory protein misc_feature 95..97 /label=start misc_feature 1175..1177 /label=stop misc_feature 1178..1180 /label=stop CDS 95..1180 /label=BBa_K143033 RBS 1181..1192 /label=rbs RBS 1181..1192 /label=BBa_K143021 CDS 1193..1912 /label=GFP protein CDS 1193..1912 /label=BBa_E0040 stem_loop 1924..1967 /label=stem_loop misc_feature 1913..1992 /label=BBa_B0010 terminator 1913..1992 /label=BBa_B0010 stem_loop 2008..2027 /label=T7 TE misc_feature 2034..2034 /label=stop polyA_site 2028..2041 /label=polya misc_feature 2001..2041 /label=BBa_B0012 terminator 2001..2041 /label=BBa_B0012 terminator 1913..2041 /label=BBa_B0015 misc_feature 2051..2071 /label=LacI Operator promoter 2087..2091 /label=Sigma A -35 promoter 2110..2115 /label=Sigma A -10 misc_feature 2122..2142 /label=LacI Operator promoter 2042..2142 /label=BBa_K143015 RBS 2143..2154 /label=rbs RBS 2143..2154 /label=BBa_K143021 CDS 2155..3204 /label=Xylose operon regulatory protein misc_feature 2155..2157 /label=start misc_feature 3205..3207 /label=stop misc_feature 3208..3210 /label=stop CDS 2155..3210 /label=BBa_K143036 RBS 3211..3222 /label=rbs RBS 3211..3222 /label=BBa_K143021 CDS 3223..3897 /label=mrfp1 misc_feature 3904..3928 /label=Help:Barcodes CDS 3223..3928 /label=BBa_E1010 ORIGIN 1 ctaaaaaaaa tattgaaaat actgacgagg ttatataaga tgaaaataag ttagtttgtt 61 taaacaacaa actaataggt gaaaaggtgg tgaaatgaaa ccagtaacgt tatacgatgt 121 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca 181 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc 241 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc 301 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca 361 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaaac 421 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga 481 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga 541 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact 601 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt 661 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca 721 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac 781 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat 841 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc 901 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt caaccaccat 961 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca 1021 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac 1081 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 1141 ggcacgacag gtttcccgac tggaaagcgg gcagtaataa aaaggtggtg aaatgcgtaa 1201 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa 1261 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac 1321 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac 1381 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt 1441 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga 1501 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat 1561 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata 1621 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt 1681 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca 1741 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac 1801 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt 1861 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aaccaggcat 1921 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1981 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2041 actcgagggt aaatgtgagc actcacaatt cattttgcaa aagttgttga ctttatctac 2101 aaggtgtggc ataatgtgtg taattgtgag cggataacaa ttaaaggtgg tgaaatgact 2161 ggattaaata aatcaactgt ctcatcacag gtaaacacgt taatgaaaga aagtatggta 2221 tttgaaatag gtcaaggaca atcaagtggc ggaagaagac ctgtcatgct tgtttttaat 2281 aaaaaggcag gatactccgt tggaatagat gttggtgtgg attatattaa tggcatttta 2341 acagaccttg aaggaacaat cgttcttgat caataccgcc atttggaatc caattctcca 2401 gaaataacga aagacatttt gattgatatg attcatcact ttattacgca aatgccccaa 2461 tctccgtacg ggtttattgg tataggtatt tgcgtgcctg gactcattga taaagatcaa 2521 aaaattgttt tcactccgaa ctccaactgg agagatattg acttaaaatc ttcgatacaa 2581 gagaagtaca atgtgtctgt ttttattgaa aatgaggcaa atgctggcgc atatggagaa 2641 aaactatttg gagctgcaaa aaatcacgat aacattattt acgtaagtat cagcacagga 2701 atagggatcg gtgttattat caacaatcat ttatatagag gagtaagcgg cttctctgga 2761 gaaatgggac atatgacaat agactttaat ggtcctaaat gcagttgcgg aaaccgagga 2821 tgctgggaat tgtatgcttc agagaaggct ttattaaaat ctcttcagac caaagagaaa 2881 aaactgtcct atcaagatat cataaacctc gcccatctga atgatatcgg aaccttaaat 2941 gcattacaaa attttggatt ctatttagga ataggcctta ccaatattct aaatactttc 3001 aacccacaag ccgtaatttt aagaaatagc ataattgaat cgcatcctat ggttttaaat 3061 tcaatgagaa gtgaagtatc atcaagggtt tattcccaat taggcaatag ctatgaatta 3121 ttgccatctt ccttaggaca gaatgcaccg gcattaggaa tgtcctccat tgtgattgat 3181 cattttctgg acatgattac aatgtaataa aaaggtggtg aaatggcttc ctccgaagac 3241 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag 3301 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg 3361 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag 3421 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc 3481 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc 3541 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt 3601 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc 3661 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa 3721 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg 3781 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa 3841 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa 3901 taacgctgat agtgctagtg tagatcgc //