LOCUS       BBa_K1713014            2098 bp    DNA     linear       03-May-2024
DEFINITION  B0032+lacI(-LVA)+B0032+YFP+B0015
ACCESSION   BBa_K1713014
VERSION     BBa_K1713014.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    1109..1147
                     /label=LVA
     CDS             20..1147
                     /label=lacI-LVA
     misc_feature    20..1147
                     /label=BBa_C0012
     misc_feature    1148..1172
                     /label=Help:Barcodes
     CDS             20..1147
                     /label=BBa_C0012
     RBS             1181..1193
                     /label=RBS-3\Weak
     RBS             1187..1190
                     /label=RBS
     misc_feature    1181..1193
                     /label=BBa_B0032
     RBS             1181..1193
                     /label=BBa_B0032
     misc_feature    1956..1961
                     /label=stop
     misc_feature    1917..1955
                     /label=AAV
     misc_feature    1200..1961
                     /label=BBa_E0034
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
     terminator      1970..2098
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
       61 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
      121 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
      181 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
      241 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
      301 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
      361 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
      421 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
      481 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
      541 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
      601 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
      661 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
      721 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
      781 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
      841 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
      901 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
      961 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     1021 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1081 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     1141 ttaataactc tgatagtgct agtgtagatc tctactagag tcacacagga aagtactaga
     1201 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1261 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1321 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1381 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc
     1441 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1501 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1561 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1621 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1681 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1741 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1801 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1861 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc
     1921 ctgctgcaaa cgacgaaaac tacgctgctg ctgtttaata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//