LOCUS       BBa_K1714000            3074 bp    DNA     linear       17-May-2024
DEFINITION  pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator
ACCESSION   BBa_K1714000
VERSION     BBa_K1714000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3074
                     /label=BBa_K1714000
     misc_feature    1..1210
                     /label=BBa_I0500
     misc_feature    1..1079
                     /label=AraC
     promoter        1043..1114
                     /label=PC
     promoter        1080..1210
                     /label=Pbad
     RBS             1219..1230
                     /label=RBS B0034
     CDS             1237..1395
                     /label=Ag43 Signal Peptide
     CDS             1402..1467
                     /label=Thanatin as alpha domain
     CDS             1474..2945
                     /label=Ag43 beta domain
     stem_loop       2946..3074
                     /label=double terminator
     misc_feature    1219..1230
                     /label=BBa_B0034
     misc_feature    2946..3074
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc
     1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc
     1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc
     1381 ccggtgctgg ctgctagatc cggcagcaaa aaaccggtgc cgattattta ttgcaaccgt
     1441 cgtaccggca aatgccagcg tatgatcaga tctacgaatg tcactctcgc ctccggtgcc
     1501 acctggaata tccccgataa cgccacggtg cagtcggtgg tggatgacct cagccatgcc
     1561 ggacagattc atttcacctc cacccgcaca gggaagttcg taccggcaac cctgaaagtg
     1621 aaaaacctga acggacagaa tggcaccatc agcctgcgtg tacgcccgga tatggcacag
     1681 aacaatgctg acagactggt cattgacggc ggcagggcaa ccggaaaaac catcctgaac
     1741 ctggtgaacg ccggcaacag tgcgtcgggg ctggcgacca gcggtaaggg tattcaggtg
     1801 gtggaagcca ttaacggtgc caccacggag gaaggggcct ttgtccaggg gaacaggctg
     1861 caagccggtg cctttaacta ctccctcaac cgggacagtg atgagagctg gtatctgcgc
     1921 agtgaaaatg cttatcgtgc agaagtcccc ctgtatgcct ccatgctgac acaggcaatg
     1981 gactatgacc ggattgtggc aggctcccgc agccatcaga ccggtgtaaa tggtgaaaac
     2041 aacagcgtcc gtctcagcat tcagggcggt catctcggtc acgataacaa tggcggtatt
     2101 gcccgtgggg ccacgccgga aagcagcggc agctatggat tcgtccgtct ggagggtgac
     2161 ctgatgagaa cagaggttgc cggtatgtct gtgaccgcgg gggtatatgg tgctgctggc
     2221 cattcttccg ttgatgttaa ggatgatgac ggctcccgtg ccggcacggt ccgggatgat
     2281 gccggcagcc tgggcggata cctgaatctg gtacacacgt cctccggcct gtgggctgac
     2341 attgtggcac agggaacccg ccacagcatg aaagcgtcat cggacaataa cgacttccgc
     2401 gcccggggct ggggctggct gggctcactg gaaaccggtc tgcccttcag tatcactgac
     2461 aacctgatgc tggagccaca actgcaatat acctggcagg gactttccct ggatgacggt
     2521 aaggacaacg ccggttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggt
     2581 ttccgtctgg gcagccacaa cgatatgacc tttggcgaag gcacctcatc ccgtgccccc
     2641 ctgcgtgaca gtgcaaaaca cagtgtgagt gaattaccgg tgaactggtg ggtacagcct
     2701 tctgttatcc gcaccttcag ctcccgggga gatatgcgtg tggggacttc cactgcaagc
     2761 agcgggatga cgttctctcc ctcacagaat ggcacatcac tggacctgca agccggactg
     2821 gaagcccgtg tccgggaaaa tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc
     2881 agcggcagca gcgctgaagg gtataacggt caggccacac tgaatgtgac cttctgatac
     2941 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     3001 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     3061 tttctgcgtt tata
//