LOCUS       BBa_K1714005            3146 bp    DNA     linear       10-Jun-2024
DEFINITION  pBad/araC-RBS B0034-Ag43 thanatin 2mer as alpha domain-double 
            terminator
ACCESSION   BBa_K1714005
VERSION     BBa_K1714005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3074
                     /label=BBa_K1714005
     misc_feature    1..1210
                     /label=BBa_I0500
     misc_feature    1..1079
                     /label=AraC
     promoter        1043..1114
                     /label=PC
     promoter        1080..1210
                     /label=Pbad
     RBS             1219..1230
                     /label=RBS B0034
     CDS             1237..1395
                     /label=Ag43 Signal Peptide
     CDS             1402..1539
                     /label=Thanatin 2mer as alpha domain
     stem_loop       3017..3146
                     /label=double terminator
     CDS             1546..3016
                     /label=Ag43 beta domain
     misc_feature    1219..1230
                     /label=BBa_B0034
     misc_feature    3017..3146
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga aacgacatct gaatacctgc
     1261 tacaggctgg tatggaatca catgacgggc gctttcgtgg ttgcctccga actggcccgc
     1321 gcacggggta aacgtggcgg tgtggcggtt gcactgtctc ttgccgcagt cacgtcactc
     1381 ccggtgctgg ctgctagatc cggcagcaaa aaaccggtgc cgattattta ttgcaaccgt
     1441 cgtaccggca aatgccagcg tatgatcaga tccggcagca aaaaaccggt gccgattatt
     1501 tattgcaacc gtcgtaccgg caaatgccag cgtatgatca gatctacgaa tgtcactctc
     1561 gcctccggtg ccacctggaa tatccccgat aacgccacgg tgcagtcggt ggtggatgac
     1621 ctcagccatg ccggacagat tcatttcacc tccacccgca cagggaagtt cgtaccggca
     1681 accctgaaag tgaaaaacct gaacggacag aatggcacca tcagcctgcg tgtacgcccg
     1741 gatatggcac agaacaatgc tgacagactg gtcattgacg gcggcagggc aaccggaaaa
     1801 accatcctga acctggtgaa cgccggcaac agtgcgtcgg ggctggcgac cagcggtaag
     1861 ggtattcagg tggtggaagc cattaacggt gccaccacgg aggaaggggc ctttgtccag
     1921 gggaacaggc tgcaagccgg tgcctttaac tactccctca accgggacag tgatgagagc
     1981 tggtatctgc gcagtgaaaa tgcttatcgt gcagaagtcc ccctgtatgc ctccatgctg
     2041 acacaggcaa tggactatga ccggattgtg gcaggctccc gcagccatca gaccggtgta
     2101 aatggtgaaa acaacagcgt ccgtctcagc attcagggcg gtcatctcgg tcacgataac
     2161 aatggcggta ttgcccgtgg ggccacgccg gaaagcagcg gcagctatgg attcgtccgt
     2221 ctggagggtg acctgatgag aacagaggtt gccggtatgt ctgtgaccgc gggggtatat
     2281 ggtgctgctg gccattcttc cgttgatgtt aaggatgatg acggctcccg tgccggcacg
     2341 gtccgggatg atgccggcag cctgggcgga tacctgaatc tggtacacac gtcctccggc
     2401 ctgtgggctg acattgtggc acagggaacc cgccacagca tgaaagcgtc atcggacaat
     2461 aacgacttcc gcgcccgggg ctggggctgg ctgggctcac tggaaaccgg tctgcccttc
     2521 agtatcactg acaacctgat gctggagcca caactgcaat atacctggca gggactttcc
     2581 ctggatgacg gtaaggacaa cgccggttat gtgaagttcg ggcatggcag tgcacaacat
     2641 gtgcgtgccg gtttccgtct gggcagccac aacgatatga cctttggcga aggcacctca
     2701 tcccgtgccc ccctgcgtga cagtgcaaaa cacagtgtga gtgaattacc ggtgaactgg
     2761 tgggtacagc cttctgttat ccgcaccttc agctcccggg gagatatgcg tgtggggact
     2821 tccactgcaa gcagcgggat gacgttctct ccctcacaga atggcacatc actggacctg
     2881 caagccggac tggaagcccg tgtccgggaa aatatcaccc tgggcgttca ggccggttat
     2941 gcccacagcg tcagcggcag cagcgctgaa gggtataacg gtcaggccac actgaatgtg
     3001 accttctgat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     3061 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     3121 ttcgggtggg cctttctgcg tttata
//