LOCUS       BBa_K1717000            1341 bp    DNA     linear       10-Jun-2024
DEFINITION  Keratinase A under control of LacI promoter.
ACCESSION   BBa_K1717000
VERSION     BBa_K1717000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             229..1341
                     /label=BBa_K1717171
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa atacctgctg
      241 ccgaccgctg ctgctggtct gctgctcctc gctgcccagc cggcgatggc cgctcaaccg
      301 gcgaaaaatg ttgaaaagga ttatattgtc ggatttaagt caggagtgaa aaccgcatct
      361 gtcaaaaagg acatcatcaa agagagcggc ggaaaagtgg acaagcagtt tagaatcatc
      421 aacgcggcaa aagcgaagct agacaaagaa gcgcttaagg aagtcaaaaa tgatccggat
      481 gtcgcttatg tggaagagga tcatgtggcc catgccttgg cgcaaaccgt tccttacggc
      541 attcctctca ttaaagcgga caaagtgcag gctcaaggct ttaagggagc gaatgtaaaa
      601 gtagccgtcc tggatacagg aatccaagct tctcatccgg acttgaacgt agtcggcgga
      661 gcaagctttg tggctggcga agcttataac accgacggca acggacacgg cacacatgtt
      721 gccggtacag tagctgcgct tgacaataca acgggtgtat taggcgttgc gccaagcgta
      781 tccttgtacg cggttaaagt actgagttca agcggaagcg gatcatacag cggcattgta
      841 agcggaatcg agtgggcgac aacaaacggc atggatgtta tcaatatgag ccttggggga
      901 gcatcaggct cgacagcgat gaaacaggca gtcgacaatg catatgcaag aggggttgtc
      961 gttgtagctg caacagggaa cagcggatct tcaggaaaca cgaatacaat tggctatcct
     1021 gcgaaatacg attctgtcat cgctgttggt gcggtagact ctaacagcaa cagagcttca
     1081 ttttccagtg tgggagcaga gcttgaagtc atggctcctg gcgcaggcgt atacagcact
     1141 tacccaacga acacttatgc aacattgaac ggaacgtcaa tggtttctcc tcatgtagcg
     1201 ggagcagcag ctttgatctt gtcaaaacat ccgaaccttt cagcttcaca agtccgcaac
     1261 cgtctctcca gcacggcgac ttatttggga agctccttct actatgggaa aggtctgatc
     1321 aatgtcgaag ctgccgctca a
//