LOCUS       BBa_K1717173            1347 bp    DNA     linear       09-Jun-2024
DEFINITION  Keratinase US under control of LacI promoter.
ACCESSION   BBa_K1717173
VERSION     BBa_K1717173.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             229..1347
                     /label=BBa_K1717172
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa atacctgctg
      241 ccgaccgctg ctgctggtct gctgctcctc gctgcccagc cggcgatggc cgaaagggtc
      301 tccaaaacag atagtgaaaa aagctatatt gttggtttta aagcctctgc caccacaaac
      361 agctctaaga aacaagctgt cattcaaaat ggtggaaaac tagaaaaaca ataccgcctc
      421 attaatgctg cacaagtgac aatgtccgaa caagccgcca agaaacttga acatgaccct
      481 agcattgctt acgtagaaga agaccataaa gcagaagcat atgcacaaac cgtcccttat
      541 ggaatccctc aaatcaaaga accagctgta cacgctcaag gttataaagg tgctaatgtc
      601 aaagtagctg tccttgatac tggaatccac gctgcacacc ctgacttaaa tgttgcaggg
      661 ggtgcgagct tcaaaccttc agagccaaat gccacccaag actttcaatc acatgcaact
      721 cacgtagcag gaaccattgc tgcccttgat aacacaattg gtgttcttgg ggtcgctcca
      781 agcgcttccc tatatgctgt gaaagtatta gaccgtaatg gcgacggaca agacagctgg
      841 attattagcg gtattgaatg ggctgtagcg aataacatgg atgtcatcaa tatgagctta
      901 ggcggaccaa acggctccac agcgcttaaa aatgctgttg acacagcgaa taaccgcgga
      961 gtagttgtcg ttgccgcagc aggaaattca ggttcatttg gctctactag caccgttggc
     1021 tatccagcaa aatacgattc tacaggggct gttgccaatg taaactctaa caatgtcaat
     1081 aactcatctt ctagcgcagg tcctgaatta gatgtttctg cacctggtac ttctatttta
     1141 agtacggtgc caagcagtgg atacacttct tatactgcaa catctatggc gtctcctcat
     1201 gtagcaggag cagcagcggt tatcctttct aagtatccga atctaacaaa ttctcaggtt
     1261 cgccagcgct tagaaaacac ggcaacaccg cttggtgact cattctatta cggaaaaggg
     1321 ttaatcaatg ttcaagcggc ttctaac
//