BBa_K1718005 1 BBa_K1718005 LuxI and GFP behind a Bxb1 compatible genetic switch 2015-09-14T11:00:00Z 2015-09-18T07:12:53Z The luxI and GFP come from the distribution kit. See BBa_K1718002 for more details on the switch. The switch is made up of a strong constitutive promoter followed by a terminator. The terminator is flanked by attB and attP so when in the presence of the Bxb1 invertase, the terminator will be inverted, allowing for expression of LuxI and GFP. LuxI is necessary for the production of AHL, a signaling molecule. true false _2138_ 26137 22016 9 false When on a high copy plasmid (pSB1C3), there was some leaky expression; therefore, we recommend that this part be used on plasmids with a lower copy number. false Michael Donovan component2456635 1 BBa_K1718002 component2456653 1 BBa_K1718004 annotation2456635 1 BBa_K1718002 range2456635 1 1 293 annotation2456653 1 BBa_K1718004 range2456653 1 302 1851 BBa_K1718004 1 BBa_K1718004 LuxI with GFP 2015-09-14T11:00:00Z 2015-09-15T02:15:57Z All parts were taken from the iGEM 2015 distribution kit. This part contains the LuxI gene with an RBS followed by a GFP reporter. false false _2138_ 22016 22016 9 false An RBS was added in front of the LuxI coding region. false Michael Donovan annotation2456615 1 BBa_C0061 range2456615 1 19 636 annotation2456618 1 BBa_B0034 range2456618 1 670 681 annotation2456623 1 BBa_B0012 range2456623 1 1504 1544 annotation2456627 1 BBa_I13504 range2456627 1 670 1544 annotation2456613 1 BBa_B0034 range2456613 1 1 12 annotation2456617 1 Help:Barcodes range2456617 1 637 661 annotation2456626 1 stop range2456626 1 1537 1537 annotation2456614 1 luxI range2456614 1 19 597 annotation2456621 1 BBa_B0010 range2456621 1 1416 1495 annotation2456630 1 RBS region range2456630 1 1 24 annotation2456616 1 LVA range2456616 1 598 629 annotation2456625 1 polya range2456625 1 1531 1544 annotation2456612 1 conserved range2456612 1 5 8 annotation2456622 1 stem_loop range2456622 1 1427 1470 annotation2456624 1 T7 TE range2456624 1 1511 1530 annotation2456619 1 conserved range2456619 1 674 677 annotation2456620 1 BBa_E0040 range2456620 1 688 1407 BBa_K1718002 1 BBa_K1718002 Genetic Switch for Bxb1 invertase 2015-09-12T11:00:00Z 2015-09-15T02:08:17Z Sequences are based on the Endy experiments. This part consists of a strong Anderson promoter and a terminator flanked by attB and attP, the recognition sites for BxbI. In its off position, the terminator prevents expression of the downstream part. If the BxbI invertase is expressed, it will recognize the attB and attP sites and invert the DNA; thus, flipping the terminator so polymerase can pass through the gate. The Bxb1 invertase flips DNA using the attB and attP sites. There is a Bxb1 excisionase that can flip the DNA back. false false _2138_ 0 22016 22016 9 It's complicated false The design consists of a terminator to stop transcription from the strong constitutive promoter. false Daren Kraft and Josephina Hendrix BBa_K1718005_sequence 1 aaaaaatttatttgctttcgcatctttttgtacctataatgtgtggaaagcttagctagctcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggcccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaagggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgaagctgtcaccggatgtgtactagagttcattaaagaggagaaaggtaccatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1718004_sequence 1 ttcattaaagaggagaaaggtaccatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1718002_sequence 1 aaaaaatttatttgctttcgcatctttttgtacctataatgtgtggaaagcttagctagctcggccggcttgtcgacgacggcggtctccgtcgtcaggatcatccgggcccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaagggtttgtaccgtacaccactgagaccgcggtggttgaccagacaaaccacgaagctgtcaccggatgtg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z