LOCUS       BBa_K1719012            1375 bp    DNA     linear       17-May-2024
DEFINITION  LacI+B0032+leudh+B1006
ACCESSION   BBa_K1719012
VERSION     BBa_K1719012.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     misc_feature    1..221
                     /label=BBa_K1719011
     CDS             228..1328
                     /label=BBa_K1719000
     misc_feature    1337..1375
                     /label=B1006
     stem_loop       1346..1367
                     /label=modified thr terminator
     polyA_site      1368..1375
                     /label=PolyA
     polyA_site      1337..1345
                     /label=PolyA
     terminator      1337..1375
                     /label=BBa_B1006
     misc_feature    228..1375
                     /label=BBa_K1719002
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg acattggaaa
      241 tcttcgaata tctggaaaag tatgattatg aacaggttgt gttttgtcag gacaaagaga
      301 gcggactgaa agcaattatc gccattcatg acactaccct gggaccggct ctcggcggta
      361 cccgcatgtg gacctatgat tcagaggaag cggcaatcga agatgcactc cgtctggcaa
      421 aaggcatgac atataaaaac gcagctgctg gtttaaatct gggtggcgcg aaaaccgtca
      481 ttattggcga tccgcgtaaa gataaatcag aagcgatgtt tcgtgccctg ggtcgctaca
      541 tccaagggct gaacgggcgt tatattaccg cggaagatgt aggcactacg gttgacgata
      601 tggatatcat tcacgaggag actgattttg tcacgggcat ctctccgtcc ttcgggagct
      661 ccggaaatcc gtctccggtt accgcttatg gggtttaccg cgggatgaaa gcggcggcga
      721 aagaggcgtt tggtaccgac aatttagagg gcaaagtgat tgcggtgcaa ggtgtgggca
      781 atgtggccta tcacctttgc aagcacctgc acgccgaggg tgcaaagtta attgtcaccg
      841 acatcaataa ggaggccgtc cagcgtgccg tagaggagtt cggtgctacg gcggtggagc
      901 ctaacgaaat ttatggggtg gaatgcgaca tttatgcgcc atgtgcactc ggcgcaacgg
      961 taaatgatga aaccatccca cagcttaaag cgaaagtgat tgccggcagc gctaataatc
     1021 agcttaaaga agatcggcat ggcgatatca ttcatgaaat gggcatcgtc tacgccccgg
     1081 attatgttat taatgcgggt ggtgttatta acgtggcgga tgaactgtac ggctataacc
     1141 gcgaacgtgc actgaaacgt gtggaaagca tttacgacac cattgccaag gtgatcgaaa
     1201 tctcgaaacg ggatggcatc gccacgtacg tagccgcgga tcggttggcg gaagaacgca
     1261 tcgcctcgtt gaaaaacagt cgctcgacgt atctgcgcaa cggccatgac atcattagtc
     1321 gccggtaata ctagagaaaa aaaaaccccg cccctgacag ggcggggttt ttttt
//