BBa_K1725420
1
UirR
UirR (UV intensity response Regulator)
2015-09-07T11:00:00Z
2015-09-08T06:52:10Z
The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803
UirR is a protein that attaches itself to the transmembrane protein UirS (K1725410). After exposure to unidirectional UV-A, UirS releases UirR, which physically interacts with the lsiR promoter (K1725400), activating transcription. All three parts are required for a cell to respond to the UV-A stimulus.
false
false
_2146_
21308
21308
9
false
Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.
false
Andrey Filipov
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_I13500
1
BBa_I13500
Screening plasmid intermediate
2005-05-30T11:00:00Z
2015-08-31T04:07:34Z
Released HQ 2013
Built by Josh as an intermediate in screening plasmid construction
false
true
_11_
0
253
6
In stock
false
true
jkm
component1505002
1
BBa_E0040
component1505000
1
BBa_B0034
annotation1505000
1
BBa_B0034
range1505000
1
1
12
annotation1505002
1
BBa_E0040
range1505002
1
19
738
BBa_K1725401
1
PlsiR.GFP
PlsiR + B0034 + GFP
2015-09-07T11:00:00Z
2015-09-08T11:23:40Z
The promoter comes from the genome of the unicellular cyanobacterium Synechocystis sp. PCC6803.
The promoter activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light is upstream of GFP with the B0034 RBS (BBa_I13500). This allows activity in response to UV-A to be measured. For the promoter to be activated UirR and UirS must be present and the cell has to be exposed to UV-A.
false
false
_2146_
21308
21308
9
false
The promoter, including the BioBrick prefix and suffix, was ordered as a G-block. It was ligated to I13500 after restriction that was followed by an Azure-A-based gel extraction.
false
Andrey Filipov
component2446999
1
BBa_K1725400
component2447003
1
BBa_I13500
annotation2446999
1
BBa_K1725400
range2446999
1
1
534
annotation2447003
1
BBa_I13500
range2447003
1
543
1280
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1725400
1
PlsiR
PlsiR (Promoter of light and stress integrating response Regulator)
2015-09-07T11:00:00Z
2015-09-08T10:25:53Z
Synechocystis sp. PCC6803
This promoter originally comes from the unicellular cyanobacterium Synechocystis sp. PCC6803. The promoter is normally off and is activated by the release of UirR (K1725420) from the transmembrane protein UirS (K1725410) in response to unidirectional UV-A light. For the promoter to be activated all three parts must be present and the cell has to be exposed to UV-A.
false
false
_2146_
21308
21308
9
false
PlsiR was ordered as a G-block gene fragment.
false
Andrey Filipov
BBa_K1725431
1
BBa_K1725431
J23110 + RBS + UirR + B0015 + PlsiR + GFP
2015-09-07T11:00:00Z
2015-09-08T01:22:59Z
UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
The UV intensity response regulator coding sequence was positioned downstream of J23110, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.
false
false
_2146_
21308
21308
9
false
J23110, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.
false
Andrey Filipov
component2447317
1
BBa_K1725401
component2447311
1
BBa_K1725427
annotation2447317
1
BBa_K1725401
range2447317
1
934
2213
annotation2447311
1
BBa_K1725427
range2447311
1
1
925
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K1725423
1
BBa_K1725423
J23110 + RBS + UirR
2015-09-07T11:00:00Z
2015-09-16T07:37:56Z
The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
The UV intensity response regulator coding sequence was placed downstream of J23110, a constitutive promoter family member.
false
false
_2146_
21308
21308
9
false
The regulator was ligated to J23110 (which came in the form of an annealed oligo) after restriction that was followed by an Azure-A-based gel extraction.
false
Andrey Filipov
component2447054
1
BBa_J23110
component2447059
1
BBa_K1725421
annotation2447059
1
BBa_K1725421
range2447059
1
44
788
annotation2447054
1
BBa_J23110
range2447054
1
1
35
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K1725427
1
BBa_K1725427
J23110 + RBS + UirR + B0015
2015-09-07T11:00:00Z
2015-09-16T07:37:13Z
The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
The UV intensity response regulator coding sequence was positioned downstream of J23110, a constitutive promoter family member, and upstream of the double terminator B0015. This allows the part to be placed in front of other parts with weaker or non-constitutive promoters.
false
false
_2146_
21308
21308
9
false
The regulator and J23110 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.
false
Andrey Filipov
component2447240
1
BBa_B0015
component2447233
1
BBa_K1725423
annotation2447240
1
BBa_B0015
range2447240
1
797
925
annotation2447233
1
BBa_K1725423
range2447233
1
1
788
BBa_K1725421
1
BBa_K1725421
RBS + UirR
2015-09-07T11:00:00Z
2016-01-25T11:31:22Z
UirR comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.
The regulator (K1725420) that participates together with PlsiR (K1725400) and UirS (K1725410) to activate a response to UV-A light has been put downstream of the RBS B0032.
false
false
_2146_
4206
21308
9
false
Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix, alongside with the RBS, were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.
false
Andrey Filipov
component2447020
1
BBa_B0032
component2447022
1
BBa_K1725420
annotation2447020
1
BBa_B0032
range2447020
1
1
13
annotation2447022
1
BBa_K1725420
range2447022
1
20
745
BBa_K1725431_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1725427_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1725401_sequence
1
cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1725421_sequence
1
tcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
BBa_K1725420_sequence
1
atggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I13500_sequence
1
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K1725423_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatggtaaccaaaatactgattgtagaggatgaaaggctagtggcccaacacattgcccaattattaaaaagtgatggctatgaaatttgtgtgatcgccagtgatggagcaacggcgctgaaaaaaattgctgagttttatccagatctagttttactagatattcgtattaaaggagagatcgacgggatagaggtggcggaacggataaaatctctttactccatccccattgtttatctcacagctttttctgatggggaaaccctggagcgggcccagaaaaccaatccccagggctatgtgattaagccttttcggcgggaacaactattatccaccgtggcgatcgccatagccaatcatcaacagcaacgaaaacctgaggaagataccctctccacgtcaacgggccattatcgcctgcaaccgactttggactatatcgaagagcatctcgaccaaggaattaccgttgaatttctggccggggcgatcggcatgagcacagcctacttttgtcgctttttccaaaaggaaatgggatgttccccctatcaatttattatccaacaacgggttgagcgggccaaagccatactactggaaagagaattgtccattagcgaagtagctttgagatgcggcttcagttcccacagtcaacttaaccaccattttcgtaatctcttgggcattaccccgaaagaatatcgtagtcgctaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1725400_sequence
1
cagtttccaagcaagatccgggcgaaaaaataaaatcgatgtcaaaaatcaactagattgtcaaaaatcgaccaggacagggttgggaatttttcctaggatgggactgtgctggtgagcgacgggctgcgaatgccattggctcaaatcccaaccttgccaaggattagacctactcctacccgcttatttttaggcaagttcttgcaggtttgaatcctaaccgacatttttgcttctaggccttggatagcgagagttacccttggccaccaaaccttggctaagtcgatgccttttggtaccccaactagcagcaaacaccagtcatcttccttgaatccttctctggtcagtcctacgggtcactgtttggagcaatgcctggggcattgagcatttcctcccatgtcaatcacaactgacagttaaaacaagggaattactcggccctaggtctccttatcccactgacaacagcattaaccgaacactgttaaagtttatctttccaaacatgcaaaggagtttccc
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z