LOCUS BBa_K173021 4121 bp DNA linear 17-May-2024 DEFINITION pdc and adhB inducible system (HSL) ACCESSION BBa_K173021 VERSION BBa_K173021.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 RBS 1070..1084 /label=RBS-1\Strong RBS 1077..1081 /label=RBS misc_feature 1070..1084 /label=BBa_B0030 RBS 1070..1084 /label=BBa_B0030 CDS 1091..2800 /label=BBa_K173016 RBS 2809..2823 /label=RBS-1\Strong RBS 2816..2820 /label=RBS misc_feature 2809..2823 /label=BBa_B0030 RBS 2809..2823 /label=BBa_B0030 CDS 2830..3984 /label=BBa_K173017 stem_loop 4004..4047 /label=stem_loop misc_feature 3993..4072 /label=BBa_B0010 terminator 3993..4072 /label=BBa_B0010 stem_loop 4088..4107 /label=T7 TE misc_feature 4114..4114 /label=stop polyA_site 4108..4121 /label=polya misc_feature 4081..4121 /label=BBa_B0012 terminator 4081..4121 /label=BBa_B0012 misc_feature 1070..4121 /label=BBa_K173003 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga 1081 gaaatactag atgtcttata ccgtggggac ctatctggca gaacgcctgg ttcaaattgg 1141 gctgaaacac cacttcgctg ttgccggtga ttataacctg gtgctgctgg acaatctgct 1201 gctgaataaa aacatggagc aagtctattg ctgtaacgaa ctgaactgtg gctttagcgc 1261 tgaaggctat gcccgtgcca aaggtgccgc cgctgctgtt gtaacctata gtgtgggtgc 1321 cctgagtgct ttcgatgcta ttggaggggc ttatgctgaa aacctgccgg taatcctgat 1381 ttctggtgcc ccgaacaata acgatcatgc cgccggtcat gtgctgcatc atgctctggg 1441 taaaaccgac tatcattatc agctggaaat ggccaaaaac attactgccg ctgccgaggc 1501 gatttatact ccggaagagg ctccggccaa aattgatcat gtcatcaaaa ccgcactgcg 1561 tgagaaaaaa ccggtctatc tggaaattgc ttgtaacatt gcctcaatgc cgtgtgccgc 1621 tcctggtccg gctagtgccc tgtttaacga tgaagcttcg gatgaagcaa gcctgaatgc 1681 tgccgttgaa gaaacgctga aattcattgc caaccgtgac aaagtcgctg tactggtggg 1741 ttctaaactg cgcgctgctg gtgctgagga agccgctgta aaattcgctg acgccctggg 1801 tggagctgta gcaaccatgg ctgccgccaa atccttcttt cctgaggaga atcctcatta 1861 tatcggcacg agctggggcg aggtttcata tccaggggtg gagaaaacca tgaaagaggc 1921 agacgccgtt atcgctctgg ctcctgtgtt caatgactat tccaccaccg ggtggactga 1981 tattcctgac ccgaaaaaac tggtgctggc tgaacctcgt tctgttgtgg tgaatggcat 2041 ccgttttccg agcgttcacc tgaaagacta tctgactcgt ctggcccaaa aagtgtccaa 2101 aaaaactggc gctctggact tctttaaaag tctgaatgcc ggggaactga aaaaagctgc 2161 tccggccgat ccttctgctc ctctggttaa tgccgagatt gctcgtcaag ttgaagctct 2221 gctgactccg aataccaccg ttatcgccga aactggtgat tcctggttca atgcccagcg 2281 tatgaaactg ccgaatggtg ctcgtgttga gtatgagatg caatggggcc atattggttg 2341 gtctgtgcct gctgcttttg gttatgccgt tggtgctcct gaacgtcgta acatcctgat 2401 ggtaggggat ggatcatttc aactgaccgc ccaggaagtt gctcaaatgg ttcgtctgaa 2461 actgcctgtg atcatctttc tgattaacaa ctatggctat acgatcgagg tcatgattca 2521 cgatggtccg tataacaaca ttaaaaactg ggactatgct ggtctgatgg aggtgttcaa 2581 tggtaatggc gggtatgatt ctggagccgg gaaaggcctg aaagcgaaaa ccgggggtga 2641 gctggctgag gctattaaag ttgccctggc caatacagat gggcctaccc tgatcgaatg 2701 ctttattggg cgtgaggact gtacagaaga actggtaaaa tgggggaaac gtgttgccgc 2761 tgctaacagc cgtaaaccgg tgaataaact gctgtaataa tactagagat taaagaggag 2821 aaatactaga tggcatcaag caccttttat atcccgtttg ttaatgaaat gggcgagggt 2881 tctctggaaa aagcgatcaa agacctgaat ggtagtgggt tcaaaaatgc cctgattgtg 2941 tcagacgcct tcatgaataa aagtggcgtg gtgaaacagg ttgctgatct gctgaaagca 3001 cagggtatca atagcgccgt gtatgacggt gttatgccga atccgaccgt tacagccgta 3061 ctggagggac tgaaaatcct gaaagacaac aacagcgact tcgtgatttc tctgggtggt 3121 ggttctccac atgattgtgc caaagccatt gccctggtgg ctacaaatgg aggtgaagtg 3181 aaagattatg aggggatcga caaaagcaaa aaaccggcac tgcctctgat gagcatcaat 3241 accacagcgg gtacagcatc tgagatgact cgtttctgta ttatcaccga cgaggttcgc 3301 catgtgaaaa tggccatcgt ggatcgtcat gttacaccga tggttagcgt gaatgatcct 3361 ctgctgatgg tgggtatgcc taaagggctg actgccgcta caggaatgga cgccctgact 3421 catgccttcg aagcctattc tagcaccgcc gctactccta ttaccgacgc ttgtgccctg 3481 aaagctgcct ctatgattgc caaaaatctg aaaaccgctt gtgacaacgg caaagacatg 3541 cctgctcgtg aggccatggc ctatgcccaa tttctggccg gtatggcgtt taataacgcc 3601 tctctgggct atgttcatgc tatggctcac caactgggag gctattataa cctgccacac 3661 ggcgtctgta atgctgtgct gctgcctcat gttctggcct ataatgccag cgtggttgct 3721 ggtcgcctga aagatgttgg agtggctatg ggtctggata ttgccaatct gggggacaaa 3781 gagggtgctg aagcgacaat tcaagccgtc cgtgacctgg ctgcttctat cggaatcccg 3841 gcaaacctga cagaactggg cgccaaaaaa gaagatgtgc ctctgctggc tgatcatgct 3901 ctgaaagacg cttgtgctct gactaatcct cgtcagggag atcagaaaga agtcgaggag 3961 ctgtttctga gtgccttttg ataatactag agccaggcat caaataaaac gaaaggctca 4021 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 4081 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //