LOCUS       BBa_K173023             2937 bp    DNA     linear       17-May-2024
DEFINITION  pdc inducible system (HSL)
ACCESSION   BBa_K173023
VERSION     BBa_K173023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1084
                     /label=RBS-1\Strong
     RBS             1077..1081
                     /label=RBS
     misc_feature    1070..1084
                     /label=BBa_B0030
     RBS             1070..1084
                     /label=BBa_B0030
     CDS             1091..2800
                     /label=BBa_K173016
     stem_loop       2820..2863
                     /label=stem_loop
     misc_feature    2809..2888
                     /label=BBa_B0010
     terminator      2809..2888
                     /label=BBa_B0010
     stem_loop       2904..2923
                     /label=T7 TE
     misc_feature    2930..2930
                     /label=stop
     polyA_site      2924..2937
                     /label=polya
     misc_feature    2897..2937
                     /label=BBa_B0012
     terminator      2897..2937
                     /label=BBa_B0012
     misc_feature    1070..2937
                     /label=BBa_K173018
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ttaaagagga
     1081 gaaatactag atgtcttata ccgtggggac ctatctggca gaacgcctgg ttcaaattgg
     1141 gctgaaacac cacttcgctg ttgccggtga ttataacctg gtgctgctgg acaatctgct
     1201 gctgaataaa aacatggagc aagtctattg ctgtaacgaa ctgaactgtg gctttagcgc
     1261 tgaaggctat gcccgtgcca aaggtgccgc cgctgctgtt gtaacctata gtgtgggtgc
     1321 cctgagtgct ttcgatgcta ttggaggggc ttatgctgaa aacctgccgg taatcctgat
     1381 ttctggtgcc ccgaacaata acgatcatgc cgccggtcat gtgctgcatc atgctctggg
     1441 taaaaccgac tatcattatc agctggaaat ggccaaaaac attactgccg ctgccgaggc
     1501 gatttatact ccggaagagg ctccggccaa aattgatcat gtcatcaaaa ccgcactgcg
     1561 tgagaaaaaa ccggtctatc tggaaattgc ttgtaacatt gcctcaatgc cgtgtgccgc
     1621 tcctggtccg gctagtgccc tgtttaacga tgaagcttcg gatgaagcaa gcctgaatgc
     1681 tgccgttgaa gaaacgctga aattcattgc caaccgtgac aaagtcgctg tactggtggg
     1741 ttctaaactg cgcgctgctg gtgctgagga agccgctgta aaattcgctg acgccctggg
     1801 tggagctgta gcaaccatgg ctgccgccaa atccttcttt cctgaggaga atcctcatta
     1861 tatcggcacg agctggggcg aggtttcata tccaggggtg gagaaaacca tgaaagaggc
     1921 agacgccgtt atcgctctgg ctcctgtgtt caatgactat tccaccaccg ggtggactga
     1981 tattcctgac ccgaaaaaac tggtgctggc tgaacctcgt tctgttgtgg tgaatggcat
     2041 ccgttttccg agcgttcacc tgaaagacta tctgactcgt ctggcccaaa aagtgtccaa
     2101 aaaaactggc gctctggact tctttaaaag tctgaatgcc ggggaactga aaaaagctgc
     2161 tccggccgat ccttctgctc ctctggttaa tgccgagatt gctcgtcaag ttgaagctct
     2221 gctgactccg aataccaccg ttatcgccga aactggtgat tcctggttca atgcccagcg
     2281 tatgaaactg ccgaatggtg ctcgtgttga gtatgagatg caatggggcc atattggttg
     2341 gtctgtgcct gctgcttttg gttatgccgt tggtgctcct gaacgtcgta acatcctgat
     2401 ggtaggggat ggatcatttc aactgaccgc ccaggaagtt gctcaaatgg ttcgtctgaa
     2461 actgcctgtg atcatctttc tgattaacaa ctatggctat acgatcgagg tcatgattca
     2521 cgatggtccg tataacaaca ttaaaaactg ggactatgct ggtctgatgg aggtgttcaa
     2581 tggtaatggc gggtatgatt ctggagccgg gaaaggcctg aaagcgaaaa ccgggggtga
     2641 gctggctgag gctattaaag ttgccctggc caatacagat gggcctaccc tgatcgaatg
     2701 ctttattggg cgtgaggact gtacagaaga actggtaaaa tgggggaaac gtgttgccgc
     2761 tgctaacagc cgtaaaccgg tgaataaact gctgtaataa tactagagcc aggcatcaaa
     2821 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2881 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
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