Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Donna Lee
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-18 01:01:25
J23100-PelB-GlucHCO-B0015
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1732000_sequence (Version 1) |
Description
J23100 Promoter with a RBS (BBa_S04423), PelB Leader sequence, Gaussia princeps luciferase, and a B0015 terminator. Allows Gaussia princeps luciferase to be transcribed and translated using a strong promoter. Luminescence can be expressed by reacting the luciferase product with coelenterazine.Notes
This sequence is codon optimized for E.coli. Sequence contains a PelB leader sequence that allows the Gaussia princeps luciferase proteins to secreted into the periplasmic space of the cell.Source
Engineered from XXXXXXXX. Provided by the Bruchez lab at Carnegie Mellon.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K208004 BBa_K1732027 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K208004 BBa_K1732027 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K208004 BBa_K1732027 BBa_B0015 | 1,35 44,55 62,127 136,657 666,794 | BBa_J23100 BBa_B0034 BBa_K208004 BBa_K1732027 BBa_B0015 |