LOCUS       BBa_K1741004             914 bp    DNA     linear       01-Jun-2024
DEFINITION  sfGFP under melibiose promoter (improved 5'UTR)
ACCESSION   BBa_K1741004
VERSION     BBa_K1741004.1  
FEATURES             Location/Qualifiers
     misc_feature    28..45
                     /label=MelR binding site 1
     misc_feature    48..63
                     /label=CRP binding site
     misc_feature    66..84
                     /label=MelR binding site 1
     misc_feature    102..107
                     /label=-35 signal
     misc_feature    125..129
                     /label=-10 signal
     misc_feature    138..164
                     /label=improved 5'UTR
     RBS             151..156
                     /label=RBS
     misc_feature    165..194
                     /label=His-tag
     CDS             195..908
                     /label=sfGFP
     misc_feature    909..914
                     /label=two consecutive STOP codons
ORIGIN
        1 gccgaaaact ctgcttttca ggtaatttat tcccataaac tcagatttac tgctgcttca
       61 cgcaggatct gagtttatgg gaatgctcaa cctggaagcc ggaggttttc tacagattcg
      121 cctgccatga tgaagttatt caagcaagcc aggagatata tcatatgggc agcagccatc
      181 atcatcatca tcatatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
      241 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
      301 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
      361 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
      421 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
      481 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
      541 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
      601 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
      661 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
      721 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
      781 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
      841 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
      901 tgtacaaata ataa
//