LOCUS       BBa_K1741014             908 bp    DNA     linear       01-Jun-2024
DEFINITION  sfGFP under proD promoter
ACCESSION   BBa_K1741014
VERSION     BBa_K1741014.1  
FEATURES             Location/Qualifiers
     promoter        1..101
                     /label=proD promoter
     misc_feature    63..68
                     /label=-35 signal
     misc_feature    86..91
                     /label=-10 signal
     misc_feature    102..158
                     /label=5'UTR
     misc_feature    159..188
                     /label=His-tag
     CDS             189..902
                     /label=sfGFP
     misc_feature    903..908
                     /label=two consecutive STOP codons
ORIGIN
        1 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
       61 actttacggg catgcataag gctcgtataa tatattcagg gagaccacaa cggtttccct
      121 ctacaaataa ttttgtttaa ctttgaggag gttatcatat gggcagcagc catcatcatc
      181 atcatcatat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
      241 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
      301 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gtaccttggc
      361 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
      421 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
      481 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
      541 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc aatatcctgg
      601 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
      661 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
      721 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
      781 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
      841 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
      901 aataataa
//