BBa_K1741014 1 BBa_K1741014 sfGFP under proD promoter 2015-09-16T11:00:00Z 2015-09-17T07:06:25Z All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section. A strong insulated constitutive promoter proD is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend on E. coli growth rate. false false _2163_ 26053 26053 9 false None. false Marta Żardecka annotation2467543 1 His-tag range2467543 1 159 188 annotation2467545 1 two consecutive STOP codons range2467545 1 903 908 annotation2467544 1 sfGFP range2467544 1 189 902 annotation2467542 1 5'UTR range2467542 1 102 158 annotation2467539 1 proD promoter range2467539 1 1 101 annotation2467541 1 -10 signal range2467541 1 86 91 annotation2467540 1 -35 signal range2467540 1 63 68 BBa_K1741014_sequence 1 cacagctaacaccacgtcgtccctatctgctgccctaggtctatgagtggttgctggataactttacgggcatgcataaggctcgtataatatattcagggagaccacaacggtttccctctacaaataattttgtttaactttgaggaggttatcatatgggcagcagccatcatcatcatcatcatatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z